离子液体反胶团中青霉素酰化酶的水解反应特性

反胶团是表面活性剂在非极性溶剂中自聚形成的微观结构．其亲水头部朝内，疏水尾部朝外，形成可容纳极性溶质的水相空腔，在蛋白质萃取及酶催化方面得到广泛研究．对于用青霉素酰化酶水解青霉素生产6-氨基青霉烷酸（6-APA）的工艺，反胶团兼具高效性与分离便利的优点Ⅲ．目前反胶团的调控主要集中在水含量、pH值与阴离子类型等方面，对表面活性剂头部的作用还缺乏了解．

Penicillin acylase hydrolysis of 6-APA in reversed micelle with ionic liquids

Penicillin hydrolysis by penicillin acylase was carried out in reversed micelle with C_14mim]Cl ionic liquids. The experi ment indicated that this catalytic reaction showed better performance in enzymat ic solubility, activity and stability than the reaction in reversed micelle with cetyl trimethyl ammonium bromide (CTAB). For example, the maximum solubility of penicillin acylase in ionic liquids system was twice more than that in CTAB, an d the maximal enzymatic activity in ionic liquids was 1.3 times than that in CT AB.The outstanding properties of ionic liquids, including low charge density and irregular sharpness of head ring,might be attributable to its distinct head gro up. Ionic liquids made it possible to optimize the enzymatic catalysis in rever sed micelle through altering its interfacial structure effectively.