| 一株耐温耐盐烃降解菌Geobacillus sp. XDF-4性能 |
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| 引用本文： | 夏文杰,董汉平,俞理,黄立信,赵婷.一株耐温耐盐烃降解菌Geobacillus sp. XDF-4性能[J].化工学报,2010,61(11):2951-2959. |
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| 作者姓名： | 夏文杰 董汉平 俞理 黄立信 赵婷 |
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| 作者单位： | 中国科学院渗流流体力学研究所;中国石油勘探开发研究院廊坊分院 |
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| 摘 要： | 从大庆油田龙虎泡区块采油地层水中分离得到一株性能很好的耐盐耐温的兼性烃降解菌XDF-4,经形态观察、生理生化实验和16SrDNA基因序列分析,初步鉴定为地芽孢杆菌Geobacillus sp.。该菌在45～75℃、pH 6.5～9.0、盐的质量分数0～10%下生长良好,其最适生长温度为65℃,最适盐的质量分数为 3.0%。研究发现,该菌株能以原油为唯一碳源生长并合成生物表面活性剂, 发酵
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Characterization of a thermophilic and halotolerant strain Geobacillus sp. XDF-4 |
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| XIA Wenjie,DONG Hanping,YU Li,HUANG Lixin,ZHAO Ting.Characterization of a thermophilic and halotolerant strain Geobacillus sp. XDF-4[J].Journal of Chemical Industry and Engineering(China),2010,61(11):2951-2959. |
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| Authors: | XIA Wenjie DONG Hanping YU Li HUANG Lixin ZHAO Ting |
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| Abstract: | A strain XDF-4 was obtained by isolation from stratum water of oil recovery in Longhupao block of Daqing oil field, and identified as Geobacillus sp. by morphological observation, physiological and biochemical test and 16SrDNA gene sequence analysis. It grows well at 45—75℃, pH 6. 5—9.0 and salt concentration 0—10%, and the optimum conditions was 65℃ and salt concentration 3%. It was found that XDF-4 could utilize crude oil as sole carbon source to synthesize biosurfactant at high temperature and salinity, which could lead to decrease in surface tension of the culture medium from 68.59 mN·m-1 to 29.58 mN·m-1 after 7d zymolysis. The results from thin-layer chromatography and chromogenic reaction showed that the biosurfactant produced by XDF-4 involves three main compounds: glycosides 50.26%（mass）, lipids 28. 47%（mass） and protein 15. 35%（ | |
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