| D—异抗坏血酸产生菌的筛选及诱变育种 |
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| 引用本文： | 浙江工学院固定化细胞有机酸发酵科研组.D—异抗坏血酸产生菌的筛选及诱变育种[J].浙江工业大学学报,1991(1). |
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| 作者姓名： | 浙江工学院固定化细胞有机酸发酵科研组 |
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| 作者单位： | 浙江工学院轻工业工程系 |
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| 基金项目： | 浙江省自然科学基金资助项目 |
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| 摘 要： | 本文从85株霉菌中,筛选得到了六株青霉属D-异抗坏血酸(EA)产生菌,其中点青霉2553产酸能力较高.通过UV、DES或~(60)Coγ-射线对其单孢子进行诱变处理,并进行随机筛选和理性化筛选,获得一最高正变株,其产酸水平达14.47mg/ml,较出发菌株提高50%.
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| 关 键 词： | D-异抗坏血酸 点青霉 菌种筛选 诱变处理 |
Screening and Mutagenic Treatments of D-Isoascorbic Acid-Producing Strain |
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| The research group of organic acid fermentation by immobilized cells.Screening and Mutagenic Treatments of D-Isoascorbic Acid-Producing Strain[J].Journal of Zhejiang University of Technology,1991(1). |
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| Authors: | The research group of organic acid fermentation by immobilized cells |
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| Affiliation: | Department of Light Industry Engineering |
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| Abstract: | Six Penicillium D-isoascorbic acid (EA)-producing strains were screened out from among 85 fungi, and P. notatum 2553 turned out to be the most opwerful. By subjecting the monospores of this strain to various mutagenic agents-UV, DES or 60Cor-ray, and selecting by random and rational screening, a high positive strain was obtained, with its productivity being 14.47mg/ml, an increase of 50% compared with the original strain. |
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| Keywords: | D-Isoascorbic acid Penicillium notatum Screening Mutagenic treatment |
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