| 尿激酶酸性复合物解离的初步研究 |
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| 引用本文： | 梅乐和,王炜.尿激酶酸性复合物解离的初步研究[J].浙江大学学报(自然科学版 ),1999,33(2):129-131. |
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| 作者姓名： | 梅乐和 王炜 |
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| 作者单位： | 浙江大学生物化工系 |
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| 摘 要： | 初步研究了用盐酸羟胺，十二烷基硫酸钠，吐温，氨水等不同的化学试剂对尿激酶酸性复合物的解离作用，选择了氨水作为解离剂，确定了解离条件，以０．５ｍｏｌ氨水，解离３０ｍｉｎ为最好，此时尿激酶的总活力可提高３．６５倍。
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| 关 键 词： | 尿激酶 酸性复合物 解离 蛋白酶 |
Studies on the dissociation of acid complex compound of urokinase |
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| MEI Le he,WANG Wei,ZHU Zi qiang,HAN Zhao xiong.Studies on the dissociation of acid complex compound of urokinase[J].Journal of Zhejiang University(Engineering Science),1999,33(2):129-131. |
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| Authors: | MEI Le he WANG Wei ZHU Zi qiang HAN Zhao xiong |
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| Abstract: | The dissociation of acid complex compound of urokinase by sodium oxyammonia chloride,dodecyl sulphate,Tween 80,ammonia water was studied.Ammonia water was selected as dissociating agent and the favourable condition for dissociation was confirmed:0.5mol ammonia water and time for dissociation was 30 min.Under this favourable condition of dissociation,the activties of urokinase has increased by 3.65 times. |
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| Keywords: | urokinase acid complex compound dissociation |
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