胰蛋白酶水解鲜猪皮的条件选择

为克服酸碱法水解猪皮胶原对氨基酸的破坏,拟利用胰蛋白酶水解猪皮胶原来获得更多的氨基酸.研究发现,随着胰蛋白酶含量增加,水解度增加,当pH 7～8时,胰蛋白酶对猪皮水解的水解度达到最大值;水解反应的开始反应速度较大,30分钟后反应速度降低.胰蛋白酶水解猪皮的最佳条件为:pH 7.5,水解温度48℃,酶与猪皮比40mg/g,底物浓度25%,水解时间2h.在此条件下,猪皮的水解度可达16%.

Condition selection of the hydrolysis of pigskin with trypsin

In this work,trypsin was used to hydrolyze pigskin in order to avoid the destruction of collagen polypeptide during acid-alkali hydrolysis process.It was found that the hydrolysis degree increased with the increase of the trypsin content.The maximum hydrolysis degree of pigskin was realized at pH 7~8.The initial rate of the hydrolysis reaction was large while it decreased after reacting for 30 minutes.The optimum condition of the hydrolysis of pigskin by trypsin was as follows: pH 7.5,hydrolysis temperature 48 ℃,ratio of trypsin 40 mg/g,substrates concentration 8%,hydrolysis time 2 h.Under this condition,the hydrolysis degree of pigskin could reach 16%.