| 对产油脂酵母的细胞破碎方法及油脂提取效果的比较 |
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| 引用本文： | 孙晓璐,孙玉梅,曹芳,华艳艳,赵宗保.对产油脂酵母的细胞破碎方法及油脂提取效果的比较[J].河南工业大学学报(自然科学版),2007,28(4):67-69. |
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| 作者姓名： | 孙晓璐 孙玉梅 曹芳 华艳艳 赵宗保 |
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| 作者单位： | 1. 大连工业大学,生物与食品工程学院,辽宁,大连,116034
2. 中国科学院,大连物理化学研究所,辽宁,大连,116023 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 研究了冻融破碎法、超声波破碎法、酸热法以及冻融和酸热结合法破碎发酵性丝孢酵母(Trichosporon fermentans)细胞,并考察了乙醚-石油醚法和氯仿-甲醇法萃取细胞内油脂.实验结果表明,采用酸热法破碎细胞后以乙醚-石油醚作为萃取溶剂油脂提取效果较好,油脂得率高达29.8%.
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| 关 键 词： | 发酵性丝孢酵母 细胞破碎 油脂 提取 |
| 文章编号： | 1673-2383（2007）04-0067-03 |
| 收稿时间： | 2007-04-04 |
| 修稿时间： | 2007年4月4日 |
COMPARISON OF CELL DISRUPTION METHODS AND LIPID EXTRACTION RATE OF LIPID-PRODUCING YEAST |
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| SUN Xiao-lu,SUN Yu-mei,CAO Fang,HUA Yan-yan,ZHAO Zong-bao.COMPARISON OF CELL DISRUPTION METHODS AND LIPID EXTRACTION RATE OF LIPID-PRODUCING YEAST[J].Journal of Henan University of Technology Natural Science Edition,2007,28(4):67-69. |
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| Authors: | SUN Xiao-lu SUN Yu-mei CAO Fang HUA Yan-yan ZHAO Zong-bao |
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| Affiliation: | 1. College of Biology and Food Engineering ,Dalian Polytechnic University, Dalian 116034, China; 2. Division of Biotechnolog , Dalian Institute of Chemical Physics, CAS , Dalian 116023, China |
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| Abstract: | Trichosporon fermentans were disrupted by freeze-melt,ultrasonic wave,acid-heating method and freeze-melt & acid-heating method respectively.The lipid extraction rates of Trichosporon ferments by the ether-benzine and chloroform-methanol were compared.The results implied that the cells were disrupted by acid-heating and then extracted by the ether-benzine,by this procedure,the lipid extraction rate could reach higher level as high as 29.8%. |
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| Keywords: | Trichosporon fermentans cell disruption lipids extraction |
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