| 工业生产微生物谷氨酰胺转胺酶的分离工艺研究 |
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| 引用本文： | 柏俊华,高红亮,常忠义,杨俊.工业生产微生物谷氨酰胺转胺酶的分离工艺研究[J].河南工业大学学报(自然科学版),2005,26(1):84-87. |
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| 作者姓名： | 柏俊华 高红亮 常忠义 杨俊 |
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| 作者单位： | 1. 华东师范大学,生命科学学院,上海,200062
2. 江苏雨润食品产业集团公司,江苏,南京,210041 |
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| 摘 要： | 研究以Streptoverticillium mobaraense菌株发酵产生的谷氨酰胺转胺酶的分离工艺.通过发酵液分离处理后的收率和比酶活测定,确定超滤和乙醇沉淀的最适条件.试验结果表明:超滤的最适浓缩倍数为2.5～3.2;浓缩液pH值为8,冷乙醇按照酶与乙醇体积比1:1.5加入为最佳分离条件.
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| 关 键 词： | 谷氨酰胺转胺酶 超滤 浓缩 乙醇 沉淀 |
| 文章编号： | 1673-2383(2005)01-0084-04 |
| 修稿时间： | 2004年9月3日 |
ANALYSIS OF THE SEPARATION TECHNICS OF TRANSGLUTAMINASE IN INDUSTRIAL PRODUCTION BY MICROORGANISM |
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| BAI Jun-hua,GAO Hong-liang,CHANG Zhong-yi,YANG Yun.ANALYSIS OF THE SEPARATION TECHNICS OF TRANSGLUTAMINASE IN INDUSTRIAL PRODUCTION BY MICROORGANISM[J].Journal of Henan University of Technology Natural Science Edition,2005,26(1):84-87. |
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| Authors: | BAI Jun-hua GAO Hong-liang CHANG Zhong-yi YANG Yun |
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| Abstract: | The separation technics of transglutaminase produced by Streptoverticillium mobaraense was analyzed. By comparing the enzyme activities and the enzyme yields in different conditions, the optimum condition of ultrafiltration and deposition with organic solvent was confirmed. The results showed: the best condition of the enzyme separation with organic solvent is that the ultrafiltrate was concentrated 2.5 to 3.2 times, then adjust the pH to 8, and add 1.5V refrigerant ethanol. |
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| Keywords: | transglutaminase filtration concentrate ethanol-deposition acetone-deposition |
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