| 两种胶体金制备方法的比较研究 |
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| 引用本文： | 宋宏新,符海英,薛海燕,张玥,邹联柱.两种胶体金制备方法的比较研究[J].河南工业大学学报(自然科学版),2012,33(1):53-56. |
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| 作者姓名： | 宋宏新 符海英 薛海燕 张玥 邹联柱 |
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| 作者单位： | 陕西科技大学生命科学与工程学院,陕西西安,710021 |
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| 摘 要： | 通过控制还原剂的加入量(20 mL 0.01％四氯金酸溶液中1％柠檬酸钠和抗坏血酸添加量分别为300μL与400 μL),利用柠檬酸钠和抗坏血酸还原两种方法制备得到了平均粒径为20 nm的胶体金颗粒,并对其进行了抗体标记比较研究.结果显示:抗坏血酸还原法制备得到的胶体金标记的蛋白量为15.18 μg/mL(蛋白质量/胶体金体积,下同),高于柠檬酸钠还原法的5.07 μg/mL,间接ELISA法测得其效价(1∶800)也比柠檬酸钠还原法制备的颗粒标记物高(1∶400),这表明抗坏血酸还原法更适合于标记用胶体金颗粒的制备.
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| 关 键 词： | 柠檬酸钠还原法 抗坏血酸还原法 胶体金标记 抗体 |
COMPARISON OF TWO COLLOIDAL GOLD PREPARATION METHODS |
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| SONG Hong-xin
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FU Hai-ying
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XUE Hai-yan
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ZHANG Yue
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ZOU Lian-zhu.COMPARISON OF TWO COLLOIDAL GOLD PREPARATION METHODS[J].Journal of Henan University of Technology Natural Science Edition,2012,33(1):53-56. |
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| Authors: | SONG Hong-xin FU Hai-ying XUE Hai-yan ZHANG Yue ZOU Lian-zhu |
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| Affiliation: | (College of Life Science and Engineering,Shanxi University of Science and Technology,Xi’an 710021,China) |
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| Abstract: | In this paper,colloidal gold particles with the average particle size of 20 nm were prepared by sodium citrate reduction method and ascorbic acid reduction method by controlling the addition amount of reductants,and we also compared the antibody labeling.The results showed that the amount of protein labeled by colloidal gold prepared by ascorbic acid reduction method was 15.18 μg/mL,which was higher than that of the sodium citrate reduction method(5.07 μg/mL);and the titer of the former determined by intermediate ELISA was 1 ∶800,which was higher than that of the latter(1 ∶400).The results indicated that ascorbic acid reduction method was more suitable for the preparation of colloidal gold particles for labeling in comparison with the sodium citrate reduction method. |
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| Keywords: | sodium citrate reduction method ascorbic acid reduction method colloidal gold labeling antibody |
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