| 低强度瞬态电磁场下家猪精子的电穿孔效应 |
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| 引用本文： | 彭勇,王子淑,吴志远,郭尚勤. 低强度瞬态电磁场下家猪精子的电穿孔效应[J]. 深圳大学学报(理工版), 2003, 20(1): 36-39 |
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| 作者姓名： | 彭勇 王子淑 吴志远 郭尚勤 |
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| 作者单位： | 1. 深圳大学生命科学学院,深圳,518060
2. 四川大学生命科学学院,成都,610064 |
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| 基金项目： | 国家自然科学基金资助项目 (60 0 71 0 2 4 ) |
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| 摘 要： | 用低强度瞬态电磁场对家猪的精子细胞进行电穿孔处理 ,比较了精子细胞电穿孔前后的形态变化 .发现台盼蓝染料能够进入电穿孔后的家猪精子细胞 ,通过扫描电镜观察到电穿孔后的细胞膜表面有明显的孔洞 .实验结果表明 ,低强度瞬态电磁场能够促使猪精子细胞穿孔 ,并能促使外源物质进入细胞内部 .
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| 关 键 词： | 低强度瞬态电磁场 家猪精子 扫描电镜 电穿孔 |
| 文章编号： | 1000-2618(2003)01-0036-04 |
| 修稿时间： | 2002-03-31 |
Study of the Effects of Electroporation of Domestic Pig Sperm Cell Induced by Weak Electric Magnetic Pulse Field |
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| PENG Yong,WANG Zi-shu,WU Zhi-yuan and GUO Shang-qin)College of Life ScienceShenzhen UniversityShenzhen P.R.China). Study of the Effects of Electroporation of Domestic Pig Sperm Cell Induced by Weak Electric Magnetic Pulse Field[J]. Journal of Shenzhen University(Science &engineering), 2003, 20(1): 36-39 |
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| Authors: | PENG Yong WANG Zi-shu WU Zhi-yuan GUO Shang-qin)College of Life ScienceShenzhen UniversityShenzhen P.R.China) |
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| Affiliation: | PENG Yong~1,WANG Zi-shu~2,WU Zhi-yuan~2 and GUO Shang-qin~21)College of Life ScienceShenzhen UniversityShenzhen 518060P.R.China2)Department of BiologySichuan UniversityChengdu 610064P.R.China |
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| Abstract: | We treated the sperms of domestic pig with weak electro-magnetic pulse field and compared the shape change of the sperms.we observed that Trypan Blue can enter the sperm cells and there are obvious holes in the cell surface by scanning electron microscope. It was confirmed that weak electro-magnetic pulse field can cause electroporation of domestic pig sperms and make the little exogenous molecules enter the sperm cells. |
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| Keywords: | weak electro-magnetic pulse field pig sperm scanning electron microscope electroporation |
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