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| 竹子化学成分的测定 | |
| 引用本文： | 陈瑞,朱圣东,杨武,薛华波,唐守运.竹子化学成分的测定[J].武汉工程大学学报,2013,35(2):57-59,64. |
| 作者姓名： | 陈瑞 朱圣东 杨武 薛华波 唐守运 |
| 作者单位： | 1. 皖西学院生物与制药工程学院,安徽六安,237012 2. 武汉工程大学化工与制药学院,湖北武汉,430074 3. 安徽省植物生物技术实验实训中心,安徽六安,237012 |
| 基金项目： | 六安市定向委托皖西学院项目(2012LW001);六安市科技局的资金支持 |
| 摘 要： | 为了开发高效、低成本的预处理技术，提高竹子生物质的能源化利用，对竹子中的化学成分进行了提取和分析，并用微波和碱对竹子进行预处理和酶解试验，并设计正交试验，对经微波与碱联合预处理的竹子的糖化条件进行了优化，用硫酸蒽酮法检测酶解液中的可发酵单糖含量．其结果表明竹子中半纤维素的质量分数含量为21．49％，纤维素的质量分数含量为43．52％，可以作为生物能源生产的原料．微波与碱联合预处理的最佳条件是：700W，6min．对微波与碱联合预处理的竹屑糖化条件进行优化，得到竹屑最佳糖化条件：温度45℃，pH5．0，每克底物用酶量20U／g． |
| 关 键 词： | 纤维素 半纤维素 分析 预处理 |
Analysis of chemical components of bamboo |
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| Authors: | CHEN Rui ZHU Sheng-dong YANG Wu XUE Hua-bo TANG Shou-yun |
| Affiliation: | 1.College of Biology and Pharmaceutical Engineering,West Anhui University,Liu’an 237012,China; 2.School of Chemical Engineering and Pharmacy,Wuhan Institate of Technolgh,Wuhan 430074,China; 3.Experiment and Training Center of Plant Biotechnology of Anhui Province,Liu’an 237012,China) |
| Abstract: | Lower-cost and higher-efficiency pretreatment methods for bamboo are of great importance in energy utilization. The chemical compenents of bamboo were extracted and analyzed in this study. Enzymatic hydrolysis experiments were conducted using the bamboo which was pretreated by combining microwave irradiation with alkali. The effects of microwave irradiation power and time on saccharifi- cation were examined, and then the saccharification conditions were optimized by an orthogonal test. The results show that 100 g bamboo powder containes 43.52 g cellulose and 21.49 g hemicellulose. The optimal pretreatment conditions are 700 W, 6 min. The optimal saccharification conditions are temperature 45 ℃, pHS. 0, and enzyme loading 20 mg/g substrate. |
| Keywords: | cellulose hemicellulose analysis pretreatment |
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