等温核酸放大技术在重金属检测方面的应用 |
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引用本文: | 李婷婷,王嫦嫦,白向茹,王利华,夏 定,战艺芳,姚 琪,郑思洁.等温核酸放大技术在重金属检测方面的应用[J].武汉工程大学学报,2021,43(1):38-44. |
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作者姓名: | 李婷婷 王嫦嫦 白向茹 王利华 夏 定 战艺芳 姚 琪 郑思洁 |
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作者单位: | 武汉市农业科学院环境与安全研究所,湖北 武汉 430065 |
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摘 要: | 等温核酸放大技术是一种可以实现重金属污染物痕量分析的高灵敏性检测方法,在分析检测中有着广泛且重要的作用。本文综述了几种不同的等温核酸放大技术及其在重金属检测方面的应用,阐述分析了各方法的检测原理,为之后建立更加灵敏、快速、准确的检测方法提供了参考。虽然等温核酸放大技术目前主要停留在实验室阶段,并且检测目标有限,但是由于该技术的高灵敏性、特异性以及与其他学科紧密的联系性,具有潜在的应用前景。
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关 键 词: | 等温核酸放大技术 重金属检测 杂交链式反应 链置换扩增技术 滚环扩增技术 催化发夹组装技术 |
Application of Isothermal Nucleic Acid Amplification Technology in Heavy Metal Detection |
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Authors: | LI Tingting WANG Changchang BAI Xiangru WANG Lihua XIA Ding ZHAN Yifang YAO Qi ZHENG Sijie |
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Affiliation: | Institute of Environment and Safety, Wuhan Academy of Agricultural Sciences, Wuhan 430065,China |
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Abstract: | The isothermal nucleic acid amplification technology is a highly sensitive detection method about the target trace heavy metal, and plays an important role in the analysis and detection. In this paper, several kinds of isothermal nucleic acid amplification technologies and their application in heavy metal detection were introduced, and the detection principle of each method was analyzed, which provided a reference for the establishment of more sensitive, rapid and accurate detection methods. At present, isothermal nucleic acid amplification technology mainly stays in the laboratory stage, and the detection target is limited, but because of its high sensitivity, specificity and close connection with other disciplines, it still has the potential practical application prospects. |
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Keywords: | isothermal nucleic acid amplification technology heavy metal detection hybridization chain reaction strand displacement amplification rolling circle amplification catalytic hairpin assembly |
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