| Nutlins类似物诱导TCA-8113细胞凋亡的研究 |
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| 引用本文： | 葛雅琨,李姗姗,张元新.Nutlins类似物诱导TCA-8113细胞凋亡的研究[J].吉林化工学院学报,2014(1):10-14. |
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| 作者姓名： | 葛雅琨 李姗姗 张元新 |
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| 作者单位： | [1]吉林化工学院化工与生物技术学院,吉林吉林132022 [2]长春市口腔医院牙周病科,吉林长春130020 |
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| 基金项目： | 吉林化工学院资助项目（2011053） |
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| 摘 要： | 目的:研究新型小分子化合物Nutlins类似物在体外对舌癌TCA-8113细胞的凋亡作用.方法:采用MTT法检测新型小分子化合物Nutlins类似物对TCA-8113细胞增殖的影响;用流式细胞仪(FCM)检测Nutlins类似物诱导TCA-8113细胞凋亡情况和周期阻滞情况;用DAPI染色法观察细胞核变化.结果:Nutlins类似物NL-11和NL19以浓度依赖的方式抑制TCA-8113细胞增殖并诱导其凋亡,而且能引起TCA-8113细胞发生G2期周期阻滞.结论:NL-11和NL-19化合物能够有效地在体外抑制TCA-8113细胞增殖,并诱导细胞凋亡.
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| 关 键 词： | Nutlins类似物 舌癌 细胞凋亡 细胞周期阻滞 |
The Study of Nutlins Analogs Induced TCA-8113 Cell Apoptosis |
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| GE Ya-kun,LI Shan-shan,ZHANG Yuan-xin.The Study of Nutlins Analogs Induced TCA-8113 Cell Apoptosis[J].Journal of Jilin Institute of Chemical Technology,2014(1):10-14. |
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| Authors: | GE Ya-kun LI Shan-shan ZHANG Yuan-xin |
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| Affiliation: | 1. College of Chemical and Biological technology, Jilin Institute of Chemical Technology, Jilin City 132022, China ;2. Changchun Stomatologieal Hospital, Changchun City 130020 ,China) |
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| Abstract: | Objective：To observe the apoptosis of TCA-8113 cells induced in vitro by Nutlins analogs（NL 1 - NL 22）. Methods ： The effect of NLs on proliferation of TCA-8113 cells were determined by MTI＇ method. The apoptosis and cell cycle arrest of TCA-8113 cells were determined by flow cytometry. Results： NL-11 and 19 showed dose-dependent inhibitory effect on the proliferation of TCA-8113 cells. They induced the apoptosis and cell cycle arrest of TCA-8113 cells in dose-dependent manner and caused cell cycle arrest at G2 phase. Conclusion：NL-11 and 19 induced the apoptosis and cell cycle arrest in TCA-8113 cells in vitro. |
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| Keywords: | Nutlins analogs TCA-8113 cells cell apoptosis cell cycle arrest |
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