| 高效表达AIM工程菌的发酵培养基及发酵条件优化 |
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| 引用本文： | 黎明,张俊环,周政,牛涛.高效表达AIM工程菌的发酵培养基及发酵条件优化[J].天津轻工业学院学报,2010(3):9-13. |
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| 作者姓名： | 黎明 张俊环 周政 牛涛 |
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| 作者单位： | 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 |
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| 摘 要： | 为了进一步提高工程菌的载脂蛋白AI米兰突变体（AIM）的表达量，降低发酵成本，通过碳氮源优化实验并结合正交实验筛选出该工程菌产A1M的最适培养基为（g／L）：蔗糖10，酵母粉10，硫酸铵6和NaCl10；最适发酵条件为：装液量30mL，接种量8％，诱导时机A600 1．0，诱导剂浓度0．8mmol／L，诱导时间6h．在此优化的条件下，A1M的表达量为2．26g/L，是未优化条件下的1．58倍．
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| 关 键 词： | 载脂蛋白AI米兰突变体 发酵培养基 发酵条件 大肠杆菌 |
Optimization of Fermentation Mediums and Fermentation Conditions for AIM Production by Engineering Bacteria |
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| LI Ming,ZHANG Jun-huan,ZHOU Zheng,NIU Tao.Optimization of Fermentation Mediums and Fermentation Conditions for AIM Production by Engineering Bacteria[J].Journal of Tianjin University of Light Industry,2010(3):9-13. |
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| Authors: | LI Ming ZHANG Jun-huan ZHOU Zheng NIU Tao |
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| Affiliation: | (Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology Tianjin University of Science ＆ Technology,Tianjin 300457 ,China) |
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| Abstract: | The optimal medium and optimal fermentation condition for AIM production by engineering bacteria were founded through carbon and nitrogen sources optimization experiments and orthogonal experiments. The optimal medium （g/L） ：sucrose 10,yeast powder 10, ammonium sulfate 6 and NaCl 10. The optimal fermentation condition ：liquid loading 30 mL,inoculum size 8%,induction opportunity A600 1.0,inductor concentration 0.8 mmol/L,induction time 6 h. Expression level of AIM is 2.26 g/L in the optimized conditions, which is the 1.58 times of the expression level in the basic conditions. |
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| Keywords: | apolipoprotein A-I-Milano fermentation media fermentation conditions E coli |
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