外源调节物质对人参毛状根生长及皂苷合成的影响

通过向人参毛状根培养基中添加植物激素类物质2,4-二氯苯氧乙酸(2,4-D)、萘乙酸(NAA)、3-吲哚丁酸(IBA)、诱导子茉莉酸甲酯(MJA)和水杨酸(SA),研究了这5种外源物质对人参毛状根的生长量、总皂苷及单体皂苷Rb1的影响。结果表明,3种外源激素在适宜浓度下均能在不同程度上促进总皂苷和单体皂苷Rb1的积累。其中0.5mg/L的IBA能显著促进人参毛状根的生长(月增长量为50.73g/L)和总皂苷(质量分数为3.31%)的积累,单体皂苷Rb1(质量分数为2.293%)的含量也明显增大,使其在总皂苷中所占的比例增大。另外,2种诱导子对人参毛状根的生长无明显促进作用,在适当的浓度下能够促进人参毛状根人参皂苷的积累,并且这两种诱导子对人参皂苷含量的增加具有明显的协同作用,当MJA与SA浓度均为0.001mmol/L时,其对总皂苷和单体皂苷的积累比二者单独作用要大。

Effect of extrinsic regulators on growth and ginsenoside content of hairy roots of Panax ginseng

The effect of adding 2,4-D,NAA,IBA,MJA and SA to cell suspension cultures on the growth,content of total saponins and ginsenoside Rb1of Panax ginsenghairy roots were investigated.Results show that three phytohormones could improve the growth of the hairy roots and promote saponin accumulation in different degrees.Addition of IBA at the level of 0.5mg/L could significantly promote the growth(50.73g/L per month) ,and saponin accumulation(saponin content of 3.31%) of the hairy roots and raise remarkably the proportion of ginsenoside Rb1(Rb1content of 2.293%) in the total saponins.Other two elicitors could not significantly promote the growth,but,with suitable concentrations,could promote saponin accumulation of the hairy roots.The two elicitors have good synergistic effect on the saponin accumulation.With the combination of 0.001mmol/L MJA and 0.001mmol/L SA,the accumulation of total ginsenosides of the hairy roots is higher than that with either elicitor alone.