| 利用胶体金复合抗体提高SPR生物传感器的微生物检测精度 |
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| 引用本文： | 殷涌光,葛晶,于庆宇,王凯.利用胶体金复合抗体提高SPR生物传感器的微生物检测精度[J].吉林大学学报(工学版),2005,35(4):446-450. |
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| 作者姓名： | 殷涌光 葛晶 于庆宇 王凯 |
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| 作者单位： | 1. 吉林大学,生物与农业工程学院,长春,130022
2. 吉林大学,生物与农业工程学院,长春,130022;吉林省科技厅,长春,130000 |
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| 基金项目： | 吉林省科技厅国际合作基金资助项目(20020802) |
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| 摘 要： | 使用集成化手持式SpreetaTMSPR传感器快速检测大肠杆菌,将亲和素生物素系统用于SPR免疫传感器以保证检测的准确性。利用大肠杆菌抗体的免疫吸附反应,采用一次抗体抗原二次抗体的夹心式方法增加质量,扩大检测大肠杆菌的信号。引入胶体金复合抗体作为二次抗体大幅度增加质量,进一步扩大了检测信号。同时延长胶体金复合抗体与微生物的结合过程,使检测信号进一步稳定与放大,从而显著提高了检测精度,使该传感器对大肠杆菌的检测精度由106cfu/mL提高到101cfu/mL。
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| 关 键 词： | 仪器检验 胶体金复合抗体 SPR生物传感器 微生物 检测精度 |
| 文章编号： | 1671-5497(2005)04-0446-05 |
| 收稿时间： | 2005-02-17 |
| 修稿时间： | 2005年2月17日 |
Immune Colloid Gold to Improve Microbe Detection Precision of SPR Biosensor |
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| YIN Yong-guang,GE Jing,Yu Qing-yu,WANG Kai.Immune Colloid Gold to Improve Microbe Detection Precision of SPR Biosensor[J].Journal of Jilin University:Eng and Technol Ed,2005,35(4):446-450. |
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| Authors: | YIN Yong-guang GE Jing Yu Qing-yu WANG Kai |
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| Affiliation: | 1.College of Biological and Agricultural Engineering, Jilin University, Changchun 130022,China; 2.Departement of Science and Technology of Jilin Province, Changchun 130000,China |
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| Abstract: | A rapid assay for E.Coli 0157∶H7 has been developed by using an integrated portable Spreeta~ TMSPR biosensor. Avidin-biotin system was used to guarantee the detection accuracy. The assay is based on the adsorption of E.Coli 0157∶H7 antibody. Using the sandwich method of primary antibody-antigen-secondary antibody to increase the antigen mass and to amplify the E.Coli 0157∶H7 detection signal. Adopting immunity colloid gold as secondary antibody, the antigen mass was significantly increased and detection signal was further enhanced. At the same time, the assay has prolonged the combination time of colloid gold antibody and microbe, then the detection signal was amplified further, thereby the detection limit has been extended from 10~6 cfu/mL to 10~1 cfu/mL. |
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| Keywords: | instrument detection immune colloid gold SPR biosensor microbe detection limit |
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