| 生孢噬纤维菌CMcase基因的分离与表达 |
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| 引用本文： | 刘建,张春玉,李巍,刘东波.生孢噬纤维菌CMcase基因的分离与表达[J].长春光学精密机械学院学报,2011(4):122-124. |
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| 作者姓名： | 刘建 张春玉 李巍 刘东波 |
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| 作者单位： | [1]长春理工大学生命科学技术学院,长春130022 [2]东北师范大学生命科学学院,长春130024 |
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| 基金项目： | 国家自然科学基金项目（30670060） |
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| 摘 要： | 从生孢噬纤维菌中分离完整的基因组DNA,经过PstI限制性内切酶部分酶切后,在T4DNA连接酶作用下,将酶切的片段连接到载体pUC18的PstI位点中,然后转化到E.coli DH5α中,构建了基因组文库,经过筛选获得4.2x103个重组子。经过刚果红平板筛选获得了含有CMCase基因片段的重组子,在E.coli中进行表达,E.coli在37℃培养25~30h,培养液中CMCase活力达到22.8U/mL。
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| 关 键 词： | 生孢噬纤维菌 CMcase基因 基因组文库 表达 |
Isolation and Expression of CMcase Gene from Sporocytophaga sp.JL-01 |
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| LIU Jian,ZHANG Chunyu,LI Wei,LIU Dongbo.Isolation and Expression of CMcase Gene from Sporocytophaga sp.JL-01[J].Journal of Changchun Institute of Optics and Fine Mechanics,2011(4):122-124. |
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| Authors: | LIU Jian ZHANG Chunyu LI Wei LIU Dongbo |
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| Affiliation: | 1.chool of Life Science,Changchun University of Science and Technology,Changchun 130022; 2.School of Life Science,Northeast Normal University,Changchun 130024) |
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| Abstract: | In this study,the genomic library of Sporocytophaga sp.JL-01 was constructed.The genom DNA isolated from Sporocytophaga was partially digested with PstI,and then the fragments were ligated to pUC18 vector（PstI） and transformed into E.coli DH5α.4.2x103 recombinants were obtained by means of screening.Recombinant containing a DNA fragment of CMC cellulose（CMCase） achieved by making use of Congo red plate.The CMCase gene was expressed in E.coli.When the E.coli was cultivated at 37℃ for 25~30 hours,the cellulase activity reached 22.8U/mL. |
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| Keywords: | sporocytophaga cmcase gene genomic library expression |
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