多肽在毛细管区带电泳的迁移模型

提出了一个多肽在毛细管区带电泳的迁移模型．该模型显示出，多肽的迁移时间是其电荷、相对分子质量、构象参数（Ｒｃ）以及电泳操作条件参数（Ａ）的函数．引入的构象参数表征了生物大分子的形状多样性，由于生物大分子种类的多样性和其构象的复杂性，其构象参数应是大于１／３的实数点的集合，参数Ｒｃ和Ａ可从实验数据拟合求得，测定了在ｐＨ＝２．５的缓冲溶液中，不同实验条件下的九种标准多肽物质的毛细管区带电泳的迁移时间，实验数据与本文的理论预计一致．

A Peptides Migration Model in capillary Zone Electrophoresis

A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass (Mr), conformation parameter (Rc) of peptides and electrophoretic condition parameter(A). The conformation parameter is introduced to characterize multifarious shapes owing to the complex conlormation and the various kinds of macromolecules, where Rc≥1/3. The parameters A and Rc can be obtained from experimental data. The times of migration of the nine standard peptides were measured in pH 2.5buffer on different electrophoretic conditions in CZE. The experimental results agreed well with the theoretical prediction.