| 金黄色葡萄球菌噬菌体的分离纯化 |
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| 引用本文： | 石琰璟,郭清吉,葛晓萍,林大川.金黄色葡萄球菌噬菌体的分离纯化[J].青岛科技大学学报,2007,28(3):205-207. |
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| 作者姓名： | 石琰璟 郭清吉 葛晓萍 林大川 |
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| 作者单位： | 石琰璟（青岛科技大学,化工学院,山东,青岛,266042）;郭清吉（青岛科技大学,化工学院,山东,青岛,266042）;葛晓萍（青岛科技大学,化工学院,山东,青岛,266042）;林大川（南京农业大学,动医学院,江苏,南京,210095） |
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| 摘 要： | 分离纯化了抑制金黄色葡萄球菌的噬菌体,并对其进行效价测定。将宿主菌金黄色葡萄球菌(Staphylococcus aureus)培养制成悬液后与污水样品37℃共培养16 h,共培养物离心、过滤除菌后得到该菌的噬菌体原液。噬菌体原液采用双层平板进行3次纯化,得到直径相同的噬菌斑。纯化后金黄色葡萄球菌噬菌体的效价是8.1×108pfu。
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| 关 键 词： | 金黄色葡萄球菌 噬菌体 分离 纯化 |
| 文章编号： | 1672-6987（2007）03-0205-03 |
| 收稿时间： | 2006-10-18 |
| 修稿时间： | 2006-10-18 |
Separation and Purification of Staphylococcus Aureus Bacteriophage |
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| SHI Yan-jing,GUO Qing-ji,GE Xiao-ping,LIN Da-chuan.Separation and Purification of Staphylococcus Aureus Bacteriophage[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,2007,28(3):205-207. |
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| Authors: | SHI Yan-jing GUO Qing-ji GE Xiao-ping LIN Da-chuan |
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| Abstract: | The separation and purification of bacteriophage inhibiting Staphylococcus aureus were studied and the titre of the isolated bacteriophage was evaluated.The host strain,Staphylococcus aureus,was cultured as a suspension and then cocultured with sewage at 37℃ for 16 h,the cocultured sample was then centrifuged and filtered to eliminate bacteria and thus the bacteriophage supernatant was obtained.The bacteriophage supernatant was purified for 3 times using the two layer plates,and the bacteriophage plaques of uniform diameter were obtained.The titre of purified bacteriophage was 8.1×108pfu. |
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| Keywords: | Staphylococcus aureus bacteriophage separation purification |
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