| 亚甲基蓝与脱氧腺苷三磷酸相互作用的电化学研究 |
| |
| 引用本文： | 张旭志,焦奎,徐桂云,任勇.亚甲基蓝与脱氧腺苷三磷酸相互作用的电化学研究[J].青岛科技大学学报,2005,26(5):377-381. |
| |
| 作者姓名： | 张旭志 焦奎 徐桂云 任勇 |
| |
| 作者单位： | 青岛科技大学化学与分子工程学院 山东青岛266042
(张旭志，焦奎，徐桂云)，青岛科技大学化学与分子工程学院 山东青岛266042(任勇) |
| |
| 基金项目： | 国家自然科学基金资助项目(20375020) |
| |
| 摘 要： | 在碳糊电极上用伏安法研究了亚甲基蓝(MB)与脱氧腺苷三磷酸(dATP)的相互作用。结果表明,在B-R缓冲溶液中,随着dATP浓度的增加,MB的氧化还原峰电流降低而峰电位不变。MB和dATP形成MB-dATP非电活性复合物,结合比为1∶1,结合常数β为1.3×107。实验优化了MB与dATP的结合条件,并探讨了结合机理。
|
| 关 键 词： | 脱氧腺苷三磷酸 碳糊电极 亚甲基蓝 伏安法 |
| 文章编号： | 1672-6987(2005)05-0377-05 |
| 修稿时间： | 2005年1月6日 |
Voltammetric Studies on the Interaction of Methylene Blue with Deoxyadenosine Triphosphate |
| |
| ZHANG Xu-zhi,JIAO Kui,XU Gui-yun,REN Yong.Voltammetric Studies on the Interaction of Methylene Blue with Deoxyadenosine Triphosphate[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,2005,26(5):377-381. |
| |
| Authors: | ZHANG Xu-zhi JIAO Kui XU Gui-yun REN Yong |
| |
| Abstract: | The interaction of methylene blue(MB) with deoxyadenosine triphosphate(dATP)was studied by cyclic voltammetry on carbon paste electrode.The result indicated that dATP combined with MB in the B-R buffer solution and a new electrochemically non-active complex,MB-dATP,was formed.The peak current of MB decreased with the increase of dATP concentration.The peak potential didn't shift.The stoichiometry of MB with dATP was calculated and the result showed that the binding ratio of MB to dATP was 1∶1.The optimal conditions of the interaction between MB with dATP were studied and the mainly binding mode was electrostatic binding. |
| |
| Keywords: | deoxyadenosine triphosphate carbon paste electrode methylene blue cyclic voltammetry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
