| 负载食管癌抗原肽的脐血树突状细胞诱导杀瘤活性研究 |
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| 引用本文： | 王健,史玉,苏安英,柴锡庆,门金娥,郑海萍,张向阳,田珂. 负载食管癌抗原肽的脐血树突状细胞诱导杀瘤活性研究[J]. 河北工程大学学报(自然科学版), 2008, 25(2): 95-97 |
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| 作者姓名： | 王健 史玉 苏安英 柴锡庆 门金娥 郑海萍 张向阳 田珂 |
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| 作者单位： | 河北工程大学教务处 河北邯郸056038 |
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| 摘 要： | 为探讨负载酸洗脱食管癌抗原肽的脐血树突状细胞(DCs)对细胞毒T细胞(CTL)杀伤活性的影响,本研究采用密度梯度离心法分离脐血中的单个核细胞(CBMNC),在重组人粒巨噬细胞集落刺激因子(rhGM-CSF)、重组白介素(rhIL-4)及重组肿瘤坏死因子(rhTNF-a)的配伍作用下,以酸洗脱食管癌抗原肽负载获得分化成熟DCs,利用CytoTox 96试剂盒检测其对特异性CTL的诱导和体外杀伤效应的影响。结果:负载食管癌抗原肽的脐血DCs诱导的CTL对酸洗前食管癌细胞株T.Tn的杀伤率达(78.5±9.5)%,显著高于酸洗后组和各对照组(P<0.05),而对无关肿瘤细胞株Hep2无显著杀伤作用(P<0.05)。结论:负载食管癌抗原肽的脐血DCs体外可诱导CTL产生特异性杀伤效应。本研究为食管癌的免疫治疗提供了新的有效途经。
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| 关 键 词： | 树突状细胞 食管癌 细胞毒性T淋巴细胞 脐血 |
| 收稿时间： | 2008-03-24 |
Research of human cord blood dendritic cells loaded with peptides of esophageal carcinoma cells on cytotoxicity activity |
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| WANG Jian,SHI Yu,SU An-ying,CHAI Xi-qing,MEN Jin-e,ZHENG Hai-ping,ZHANG Xiang-yang and TIAN Ke. Research of human cord blood dendritic cells loaded with peptides of esophageal carcinoma cells on cytotoxicity activity[J]. Journal of Hebei University of Engineering(Natural Science Edition), 2008, 25(2): 95-97 |
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| Authors: | WANG Jian SHI Yu SU An-ying CHAI Xi-qing MEN Jin-e ZHENG Hai-ping ZHANG Xiang-yang TIAN Ke |
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| Affiliation: | Department of Educational Administration,Hebei University of Engineering,Handan 056038,China;Department of Educational Administration,Hebei University of Engineering,Handan 056038,China;Department of Educational Administration,Hebei University of Engineering,Handan 056038,China;Department of Educational Administration,Hebei University of Engineering,Handan 056038,China;Department of Educational Administration,Hebei University of Engineering,Handan 056038,China;Department of Educational Administration,Hebei University of Engineering,Handan 056038,China;Department of Educational Administration,Hebei University of Engineering,Handan 056038,China;Department of Educational Administration,Hebei University of Engineering,Handan 056038,China |
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| Abstract: | |
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| Keywords: | dendritic cells esophageal carcinoma cytotoxic T lymphocyte cord blood |
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