混合菌种非固定化技术制氢反应器产氢效能

为了研究混合菌种非固定化技术生物制氢反应器的产氢效能，实验采用厌氧Hungate技术和MPN法，从采用混合菌种非固定化技术的生物制氢反应器厌氧活性污染中分离到210株优势发酵菌株，其中18株为产氢细菌（HPB）。实验结果表明，主要决定反应器产氢效能的因素是反应器内HPB的数量和活性。采用混合菌种非固定化技术可以充分发挥HPB的产氢活性，但是由于反应器内HPB的数量和比例不高，大大制约了混合菌种非固定技术生物制氢反应器效能的充分发挥。针对这一结论，提出采用有自絮凝能力的高效产氢细菌进行快速启动和投加高效产氢菌株的方法提高生物制氢反应器的产氢效能。

Hydrogen production efficiency of mixed culturing bacteria in the bio- hydrogen- producing reactor with non- immobilized technology

How to enhance the hydrogen production efficiency in the bio - hydrogen - producing reactor was one of the important issues for biotechnology of hydrogen production by fermentation. For the study of efficiency in the bio - hydrogen - producing reactor, the experiments were carried out by anaerobic Hungate technology and MPB method. 210 strains of dominant fermentation bacteria were isolated from the sewage in the bio -hydrogen - producing reactor and 18 strains were hydrogen production bacteria (HPB) in these strains. The experiment result showed that the hydrogen production efficiency was determined by the number and activity of HPB. The hydrogen production activity of HPB was enhanced by the mixed culture and non - immobilized technology. But the hydrogen production efficiency was reduced because the low number and low proportion of HPB in the reactor. It was recommended that the reactor should be started up by self - flocculation HPB and the self - flocculation HPB be cast in the course of reactor running for the enhanced efficiency of hydrogen production in the reactor.