不同污泥接种物对微生物燃料电池菌群形成的影响

为考察不同接种物对微生物燃料电池效能和细菌群落形成的影响,以瓶式空气阴极微生物燃料电池为反应器,采用中美两地污水厂污泥作为接种物,分别启动氧化铟锡玻璃平板阳极微生物燃料电池,研究不同接种物对于反应器的运行效能和微生物群落结构的影响.结果表明,中国污水厂污泥启动的反应器总体运行效能要优于美国污水厂污泥启动的反应器,表现在较短的反应器启动时间,较高的运行电压、输出功率密度和电流密度及COD去除效率.两种反应器的阳极群落也显示出巨大差异,中国污水厂污泥接种的微生物燃料电池反应器,其阳极物种多样性要高于美国污水厂污泥接种的反应器.前者的微生物主要分布在5个门中,而后者的微生物仅分布在3个门.原因与中方污泥未经过氯/脱氯处理有关.利用氧化铟锡玻璃作为微生物燃料电池阳极,提取了全部的阳极生物膜进行高通量测序,从而得到更加准确的数据用于后续分析.

Impact of different sludge inoculum on microbial community formation of the microbial fuel cells

To investigate the impact of different sludge inoculum on microbial community formation of the anode in microbial fuel cells, single-chamber bottle-shaped microbial fuel cell with air cathode and planar Indium tin oxide glass anode were utilized as the experimental reactors, inoculated by sludge from Chinese and American Sewage wastewater treatment plant separately. The results indicate that the overall operation efficiency of Chinese sludge inoculated reactor is better than that of American sludge inoculated one, which shows shorter start-up time, higher operation voltage, output power density, current density, and COD removal efficiency. There are significant differences in the communities composition of the two reactors, which may be related to the fact that the one sludge is not treated by chlorination/ dechlorination. The diversity of the communities in the sludge from Chinese plant is higher than that from American plant. The communities in the former were distributed mainly in five classes. While the communities in the latter were distributed mainly the 3 classes. Using Indium tin oxide glass as the anode in microbial fuel cells could harvest the whole biofilm to do the high-throughput sequencing, resulting more accurate data to do the following analysis.