产氢发酵细菌培养基的选择和改进

高效产氢菌株的筛选与分离是生物制氢技术应用的重要基础之一，现有培养基都存在一定缺陷．为分离到高效产氢菌株，提出LM系列培养基配方，并通过产氢发酵细菌的分离和生长实验，确定了培养基的强还原剂为半胱氨酸，PH值为6．根据培养基对厌氧菌的分离数量、种类和培养基成分的分析，选择LM—1培养基为产氢发酵细菌的分离培养基．LM—1培养基分离出的5株高效产氢发酵细菌属于4个菌属，其中拟杆菌属、克雷伯氏菌属是国际上尚未分离到的高效产氢发酵细菌．利用LM—1培养基进行高效产氢细菌分离操作，得到较好的效果。

Selection and improvement of culture for hydrogen producing bacteria

LM series cultures were proposed and it was determined through bacteria isolation and experimental growth that the reducer was L-cysteine and the value of pH was 6. The number and kinds of bacteria during LM-1culture batch culturing justified the use of LM-1culture for isolation of hydrogen producing fermentation bacteria. The same conclusion was drawn from the analysis of the culture's composition. 5 isolated stains of high hydrogen producing ferment bacteria were identified as belonging to 4 genera. Among these genera, Bacteroides and Klebsiella were not belonging to the familiar genus of isolated high hydrogen producing fermentation bacteria in the world. LM-1 culture was for isolation of high effective hydrogen production bacteria, and proved highly effective.