Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum |
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Authors: | ZHANG Jun-zheng Yang Qian and Yang Lie |
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Affiliation: | Dept.of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China |
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Abstract: | Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regula-tory sequences,The pPGF plasmid was introduced by PEG/CaCl2 treatment.Positive transformants were har-Vested with hygromycin B(HYG) resistance as selective marker,and then were observed with green fluores-cence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of s.sclerotiorum.The transformants were verified mitotically stable by Southern blotting analysis and passage cultu-ring.This study is deVeloped as an initial step for further research into infection mechanisms of S.sclerotiorum to Plants and ineraetions with bio-control fungus. |
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Keywords: | sclerotinia sclerotiorum green fluorescent protein transformation hygromycin resistance gene protoplast |
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