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| 内皮细胞与小肠黏膜下层细胞相容性的初步研究 | |
| 引用本文： | 孙庆兰,张华,张西正,郭勇,李瑞欣,战锐.内皮细胞与小肠黏膜下层细胞相容性的初步研究[J].天津工业大学学报,2010,29(3). |
| 作者姓名： | 孙庆兰 张华 张西正 郭勇 李瑞欣 战锐 |
| 作者单位： | 1. 天津工业大学,理学院,天津,300160 2. 军事医学科学院,卫生装备研究所,天津,300161 3. 军事医学科学院,卫生学环境医学研究所,天津,300050 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 通过盐溶液溶胀和酶液消化制备小肠黏膜下层基质作为血管组织工程支架材料,将大鼠内皮细胞接种于支架材料上,测定小肠黏膜下层基质的细胞相容性.实验结果表明:内皮细胞在小肠黏膜下层基质上迅速铺展增殖生长,在4 h时大约有96%的细胞黏附于材料上,MTT法结果表明内皮细胞在支架上增殖生长明显,活细胞形态学观察表明细胞在支架材料上生长良好. |
| 关 键 词： | 小肠黏膜下层 支架 内皮细胞 组织工程 |
Primary research on cytocompatibility of endothelial cells and small intestinal submucosa |
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| SUN Qing-lan,ZHANG Hua,ZHANG Xi-zheng,GUO Yong,LI Rui-xin,ZHAN Rui.Primary research on cytocompatibility of endothelial cells and small intestinal submucosa[J].Journal of Tianjin Polytechnic University,2010,29(3). | |
| Authors: | SUN Qing-lan ZHANG Hua ZHANG Xi-zheng GUO Yong LI Rui-xin ZHAN Rui |
| Affiliation: | SUN Qing-lan1,ZHANG Hua1,ZHANG Xi-zheng2,GUO Yong2,LI Rui-xin2,ZHAN Rui3(1.School of Science,Tianjin Polytechnic University,Tianjin 300160,China,2.Institute of Medical Equipment,Acade-my of Military Medical Sciences,Tianjin 300161,3.Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China) |
| Abstract: | As the scaffolds of the vascular tissue engineering,the small intestinal submucosas matrix are obtained by salt solution swelling and enzyme digestion,and vascular endothelial cells are isolated from the rat thoracic aorta and subcultureed and purified.The ECs are seeded on the surface of scaffolds to determine whether the scaffolds had the cytocompatibility.Experimental results show that the seeded endothelial cells proliferate rapidly on the small intestinal submucosa matrix.About 96% of the cells adhere ... |
| Keywords: | small intestinal submucosa scaffolds endothelial cells tissue engineering |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |