| γ-亚麻酸生产菌株的诱变选育 |
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| 引用本文： | 刘阳,孟晓敏,张春枝.γ-亚麻酸生产菌株的诱变选育[J].大连工业大学学报,2006,25(3). |
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| 作者姓名： | 刘阳 孟晓敏 张春枝 |
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| 摘 要： | 采用紫外线和硫酸二乙酯对深黄被孢霉As3,3410和雅致小克银汉霉As3,2717进行诱变,筛选出一株高产γ-亚麻酸的突变株H3,3410-5。突变株H3,3410-5每升发酵液中干菌体得率为24.3 g,油脂含量为10.1 g,而出发菌株仅为21.2 g和8.4 g。气相色谱分析突变株H3,3410-5所产γ-亚麻酸的量占总脂的9.43%,出发菌株仅为8.64%。
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| 关 键 词： | γ-亚麻酸 深黄被孢霉As3 3410 雅致小克银汉霉As3 2717 诱变选育 |
Mutant breeding of gamma linolenic acid strain |
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| LIU Yang,MENG Xiao-min,ZHANG Chun-zhi.Mutant breeding of gamma linolenic acid strain[J].Journal of Dalian Dalian Polytechnic University,2006,25(3). |
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| Authors: | LIU Yang MENG Xiao-min ZHANG Chun-zhi |
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| Abstract: | The Motierella isabellina As3,3410 and Cunninghamella elegans As3,2717 were mutated by UV and diethyl sulfate.A mutant strain H3,3410-5 with high-linolenic acid yield was obtained.The dry cell weight and total lipid in H3,34105 fermentation were 24.3 g and 10.1 g per liter ferment solution,respectively.Only 21.2 g dry cell weight and 8.4 g of the total lipid in the original strain. The content of γ-linolenic acid in the total lipid of H3,3410-5 was 9.43% detected by gas chromatography,while 8.64% in the original strain. |
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| Keywords: | γ-linolenic acid Motierella isabellina As3 3410 Cunninghamella elegans As3 2717 breeding |
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