首页 | 本学科首页   官方微博 | 高级检索  
     检索      

发酵条件对短乳杆菌P-347生长和产细菌素的影响
引用本文:宫璐婵,李悦,侯英敏,孙玉梅.发酵条件对短乳杆菌P-347生长和产细菌素的影响[J].大连工业大学学报,2014(6):423-426.
作者姓名:宫璐婵  李悦  侯英敏  孙玉梅
作者单位:大连工业大学生物工程学院;
摘    要:采用单因素法,以MRS培养基为基础培养基,对短乳杆菌P-347产细菌素的适宜碳源和氮源的种类、氮源的浓度、起始pH和培养温度进行了初步探索。以金黄色葡萄球菌AS1.72为指示菌,采用牛津杯定量扩散法检测发酵上清液的抑菌活性。结果表明,短乳杆菌P-347以葡萄糖为碳源,以大豆蛋白胨为氮源,在起始pH 5.0、37℃培养24h,可获得最大菌体密度;以2%葡萄糖为碳源,以1%酪蛋白胨为氮源,在起始pH 6.5、30~37℃培养24h,发酵上清液的抑菌活性最大,抑菌圈直径为32.5mm。

关 键 词:短乳杆菌  细菌素  发酵条件

Effect of fermentation condition on the growth and the bacteriocin production by Lactobacillus brevis P-347
GONG Luchan;LI Yue;HOU Yingmin;SUN Yumei.Effect of fermentation condition on the growth and the bacteriocin production by Lactobacillus brevis P-347[J].Journal of Dalian Dalian Polytechnic University,2014(6):423-426.
Authors:GONG Luchan;LI Yue;HOU Yingmin;SUN Yumei
Institution:GONG Luchan;LI Yue;HOU Yingmin;SUN Yumei;School of Biological Engineering,Dalian Polytechnic University;
Abstract:The influence of carbon and nitrogen sources,nitrogen concentration,initial pH and temperature on cell growth and bacteriocin production of LactobacillusbrevisP-347 was investigated by single factor test.The antimicrobial activity of bacteriocin was detected against Staphylococcus aureus AS1.72 by means of the oxford plate assay system.The highest cell density could be obtained when Lactobacillus brevis P-347 was cultured in glucose and soy peptone for 24 hat 30 ℃ and initial pH 5.0.The highest antimicrobial activity in culture supernatant was obtained at 30-37 ℃ and initial pH 6.5for 24 hwith 2% glucose as carbon source and 1%casein peptone as nitrogen source,in which the inhibitory zone is 32.5mm diameter.
Keywords:Lactobacillus brevis  bacteriocin  fermentation condition
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号