采油菌株Bacillus FH-1-2的培养特性研究

以液体石蜡为碳源,对采油菌株Bacillus FH-1-2进行好氧和兼性厌氧培养。通过测定培养过程中发酵液的表面张力、菌体密度、pH值以及细胞疏水性,发现好氧和兼性厌氧培养过程中各项指标的变化趋势相近,兼性厌氧条件下的生长较慢;细胞疏水性和发酵液pH值均无显著变化;发酵液接近中性,有利于菌体生长。结果表明,细胞疏水性强能促进菌体生长。对数生长期发酵液的表面张力随菌体密度的增大而减小,在菌体生长的稳定期和衰亡期,发酵液的表面张力和菌体密度正相关。兼性厌氧培养更有利于菌株Bacillus FH-1-2应用于原油采收。

Cultivation characteristics of Bacillus FH-1-2 for MEOR

Bacillus FH-1-2 for MEOR was cultivated in liquid paraffin under aerobic and facultative anaerobic conditions.By determining the surface tension,cell density,pH of fermenting broth and hydrophobic property of cell,it was found that the fermentation trend of strain Bacillus FH-1-2 under aerobic conditions was similar to that under facultative anaerobic conditions.Cell grew slowly under facultative anaerobic.There was no significant change in hydrophobic property of cell and pH of fermenting broth during the fermentation.Fermenting broth at nearly neutral pH condition was beneficial to cell growth.The results showed that stronger hydrophobic property of cell could improve cell growth.Inverse relationship between cell density and surface tension appeared in logarithmic phase of growth,and positive relationships in both stationary phase and decay phase of growth.Bacillus FH-1-2 is suitable for MEOR under facultative anaerobic conditions.