| 绞股蓝总皂苷中单体皂苷的分离纯化 |
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| 引用本文： | 孔玉琼,金凤燮,鱼红闪.绞股蓝总皂苷中单体皂苷的分离纯化[J].大连工业大学学报,2011,30(3):173-176. |
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| 作者姓名： | 孔玉琼 金凤燮 鱼红闪 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁大连,116034 |
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| 基金项目： | 国家自然科学基金资助项目，辽宁省科学技术基金资助项目，辽宁省教育厅创新团队项目 |
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| 摘 要： | 采用硅胶柱层析法分离绞股蓝总皂苷,以氯仿-甲醇混合液为流动相进行梯度洗脱,薄层层析法跟踪检测.5 000 g绞股蓝干草经乙醇提、石油醚脱脂、正丁醇萃取、AB-8大孔吸附树脂脱糖、D-296阴离子交换树脂脱色后得到总皂苷7.87 g.5 g绞股蓝总皂苷进行硅胶柱层析分离,在体积比为9.5:0.5和9:1的氯仿-甲醇洗脱液...
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| 关 键 词： | 绞股蓝皂苷 硅胶柱层析 高效液相色谱 |
Purification of gypenoside hydrolysate |
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| KONG Yu-qiong,JIN Feng-xie,YU Hong-shan.Purification of gypenoside hydrolysate[J].Journal of Dalian Dalian Polytechnic University,2011,30(3):173-176. |
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| Authors: | KONG Yu-qiong JIN Feng-xie YU Hong-shan |
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| Affiliation: | School of Bioengineering,Dalian Polytechnic University,Dalian 116034,China |
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| Abstract: | The total gypenoside was separated and purified by silica gel column.Chloroform and methanol were used as mobile phase in gradient elution,the sample was determined by TLC.7.87 g total saponin was obtained from 5 000 g Gynostemma pentaphyllum by the process of extracted with alcohol,defatted with ligarine,leached with normal butanol,desugared with AB-8 macro porous resin,decolored with D-296 anion ion exchange resin.Three kinds of gypenoside monomer were obtained from 5 g total gypenoside by the proportion in 9.5∶0.5 and 9∶1 chloroform and methanol as mobile phase.The yields were 2.6%,3.0%,5.2% and the purity was up to 88.73%,93.11%,78.54% respectively.The result indicated that the silica gel column is a good separation function to gypenoside. |
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| Keywords: | gypenoside silica gel column HPLC |
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