| 高效液相色谱法测定伏立康唑胶囊含量研究 |
| |
| 引用本文： | 糜志远,石磊,张迎庆.高效液相色谱法测定伏立康唑胶囊含量研究[J].湖北工业大学学报,2011(5):47-49. |
| |
| 作者姓名： | 糜志远 石磊 张迎庆 |
| |
| 作者单位： | 湖北工业大学生物工程学院; |
| |
| 摘 要： | 为建立伏立康唑胶囊的高效液相色谱法含量测定方法,采用Hypersil-C18（25cm×4.6mm,10μm）色谱柱,以乙腈-0.05mol/L磷酸二氢铵溶液（pH 6.0）（V（乙腈）︰V（磷酸二氢铵溶液）=50︰50）为流动相,流速为1mL/min,进样量为20μL,检测波长为256nm.结果表明：伏立康唑在10～100mg/L浓度范围内呈现良好的线性关系,Y（峰面积）=3039.5＋4543X（浓度）,相关系数R2=0.9995.平均回收率为100.12%,相对标准偏差为1.22%.重复性试验相对标准偏差为0.80%.待测溶液在8h内稳定.本试验建立的高效液相色谱法准确度高,精密度好,能较好地用于伏立康唑胶囊含量的测定.
|
| 关 键 词： | 伏立康唑胶囊 含量测定 高效液相色谱法 |
HPLC Method for Determination of Voriconazole Capsule Content |
| |
| MI Zhi-yuan,SHI Lei,ZHANG Ying-qing.HPLC Method for Determination of Voriconazole Capsule Content[J].Journal of Hubei University of Technology,2011(5):47-49. |
| |
| Authors: | MI Zhi-yuan SHI Lei ZHANG Ying-qing |
| |
| Affiliation: | MI Zhi-yuan,SHI Lei,ZHANG Ying-qing(School of Bioengineering,Hubei University of Technology,Wuhan 430068,China) |
| |
| Abstract: | Objective: To establish the assay method of Voriconazole capsule by HPLC.Method: Hypersil-C18(25 cm×4.6 mm,10 μm) column,acetonitrile-0.05 mol/L ammonium dihydrogen phosphate aqueous solution(pH 6.0)(50:50) as mobile phase,flow rate 1 mL/min,injection volume 20 μL,UV detector,wavelength 256nm.Results: In the range of 10~100 μg/mL,good linear relationship was shown between concentration and area,Y(area)= 3039.5+4543 X(concentration),R2=0.9995,the average recovery was 100.12% with RSD 1.22%,repeatable tests RSD was 0.80%.Conclusion: HPLC method was accuracy and precise for the determination of Voriconazole capsule content. |
| |
| Keywords: | voriconazole capsule assay HPLC |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
