| 响应面法优化杏鲍菇下脚料多糖提取工艺 |
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| 引用本文： | 张庆,林凯,袁春红,李可,肖奎,向文良.响应面法优化杏鲍菇下脚料多糖提取工艺[J].西华大学学报(自然科学版),2014(2):88-92. |
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| 作者姓名： | 张庆 林凯 袁春红 李可 肖奎 向文良 |
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| 作者单位： | [1] 西华大学生物工程学院,四川 成都610039 [2] 成都榕珍菌业有限公司,四川 成都611733 |
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| 基金项目： | 四川省教育厅一般项目（13ZB0025）;西华大学重点科研基金项目（Z1220531）;榕珍菌业-西华大学产学研联合实验室（13205404） |
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| 摘 要： | 采用热水浸提法提取杏鲍菇下脚料中的多糖类物质。通过单因素试验确定液料比、提取时间和作用温度的适宜水平,并在此基础上采用Box-Behnken响应面设计法建立影响因素的二次回归数学模型,得到杏鲍菇下脚料多糖提取的最佳工艺条件为液料比26 mL/g、提取温度87℃、提取时间3.2 h,在此条件下,杏鲍菇下脚料多糖的提取率为8.76%。
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| 关 键 词： | 杏鲍菇下脚料 多糖 热水浸提 响应面分析 |
Optimization of Polysaccharide from the Residue of Pleurotus Eryngii Using Response Surface Methodology |
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| ZHANG Qing,LIN Kai,YUAN Chun-hong,LI Ke,XIAO Kui,XIANG Wen-liang.Optimization of Polysaccharide from the Residue of Pleurotus Eryngii Using Response Surface Methodology[J].Journal of Xihua University:Natural Science Edition,2014(2):88-92. |
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| Authors: | ZHANG Qing LIN Kai YUAN Chun-hong LI Ke XIAO Kui XIANG Wen-liang |
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| Affiliation: | 1. School of Bioengineering, Xihua University, Chengdu 610039 China; 2. Rong Zhen Mushrooms Industry Company Limited, Chengdu 611733 China) |
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| Abstract: | Total polysaccharides were extracted from the residue of Pleurotus eryngii by hot water extraction method .One-factor-at-a-time method and response surface methodology were used to select appropriate levels of liquid -material ratio, extraction time and temperature.A quadratic regression model describing the effects of three process conditions on the extraction rate of total polysaccha -rides was developed using a Box-Behnken experimental design .The results showed that the optimal extraction conditions were deter-mined as follows：liquid/material ratio of 26 mL/g, extraction temperature 87℃and extraction time 3.2 h.Under the optimized condi-tions, the extraction rate of polysaccharide from the residue of pleurotus eryngii reached 8.76%. |
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| Keywords: | Pleurotus eryngii residue polysaccharide hot water extraction technology response surface methodology |
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