| ρ(P)/ρ(C)动态变化对SBR强化生物除磷系统微生物种群的影响 |
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| 引用本文： | 王亚宜,江帆,张兆祥,李小伟,杨健,高乃云,彭永臻.ρ(P)/ρ(C)动态变化对SBR强化生物除磷系统微生物种群的影响[J].北京工业大学学报,2011,37(5):740-747. |
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| 作者姓名： | 王亚宜 江帆 张兆祥 李小伟 杨健 高乃云 彭永臻 |
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| 作者单位： | 同济大学污染控制与资源化研究国家重点实验室,上海,200092;北京工业大学水质科学与水环境恢复工程重点实验室,北京,100124 |
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| 基金项目： | 国家自然科学基金资助项目(5060806450628808); 全国优秀博士学位论文作者专项基金资助项目(200756); 国家教育部新世纪优秀人才支持计划资助项目(NCET-08-0404); 上海市科技人才计划资助项目(09QA1406100); 国家科技重大专项资助项目(2008ZX07421-0022008ZX07407-007-1) |
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| 摘 要： | 通过调节进水ρ(P)ρ/(C)的不同水平(2.9/100、1.4/100、0.57/100、0.29/100、1.4/100和2.9/100),考察了A/O-SBR系统强化生物除磷效果的动态变化;同时利用PCR-DGGE分子生物学技术,研究了聚磷菌和聚糖菌种群的竞争与演变.结果表明,当ρ(P)ρ/(C)逐渐降低时(2.9/100→1.4/100→0.57/100→0.29/100),吸收单位碳源的厌氧释磷量逐渐降低,而胞内糖原量逐渐升高.相应的DGGE图谱显示,微生物类群在ρ(P)/ρ(C)降低过程由11 OTUs升高到14 OTUs,最后降至7 OTUs;结合生化指标判断,系统优势菌种呈现的是从聚磷菌占优势、聚糖菌聚磷菌共存到聚糖菌占优势的动态变化.随后,提高进水ρ(P)ρ/(C)值从0.29/100到1.4/100再到2.9/100,污泥吸收单位碳源的厌氧释磷量逐渐升高,而胞内糖原量逐渐降低.这说明当聚糖菌占优势以后,通过调节ρ(P)ρ/(C)可重新培养获得聚磷菌优势系统,但DGGE图谱也显示,此时的聚磷菌优势种群较聚糖菌系统的优势种群已有较大变化,且与先前聚磷菌系统的优势种群也不尽相同.
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| 关 键 词： | 强化生物除磷 磷碳比 聚磷菌 聚糖菌 PCR-DGGE 序批式反应器 |
Influence of P/C Ratios on the Dynamic Changes of the Organism Community for the Sequencing Batch Reactor (SBR) Enhanced Biological Phosphorus Removal System |
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| WANG Ya-yi,JIANG Fan,ZHANG Zhao-xiang,LI Xiao-wei,YANG Jian,GAO Nai-yun,PENG Yong-zhen.Influence of P/C Ratios on the Dynamic Changes of the Organism Community for the Sequencing Batch Reactor (SBR) Enhanced Biological Phosphorus Removal System[J].Journal of Beijing Polytechnic University,2011,37(5):740-747. |
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| Authors: | WANG Ya-yi JIANG Fan ZHANG Zhao-xiang LI Xiao-wei YANG Jian GAO Nai-yun PENG Yong-zhen |
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| Affiliation: | WANG Ya-yi1,JIANG Fan1,ZHANG Zhao-xiang1,LI Xiao-wei1,YANG Jian1,GAO Nai-yun1,PENG Yong-zhen2(1.State Key Laboratory of Pollution Control and Resources Reuse,College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China,2.Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China) |
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| Abstract: | The effect of the different P/C ratios,i.e.,2.9/100,1.4/100,0.57/100,0.29/100,1.4/100 and 2.9/100,on the dynamic changes of the phosphorus removal as well as the organism community was examined in an anaerobic/aerobic sequencing batch reactor(A/O-SBR) system,affiliated with Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis(PCR-DGGE).Results showed that when the P/C ratio dropped(2.9/100→1.4/100→0.57/100→0.29/100) gradually,the P-release to substrate-uptake ratios decreased companied by an increase in the intracellular glycogen content.Also,the DGGE profiles showed that with the decrease of the influent P/C ratios the dominant microbial species increased from 11 operation taxonomy units(OTUs) to 14 OTUs,and then dropped to 7 OTUs.Judged from the biochemical characteristics of the SBR,it can be confirmed that the predominant microbial populations in the SBR had changed from PAOs to PAOs and GAOs co-exist,and finally to GAOs.For the last recovery period,the P-release to substrate-uptake ratios increased gradually with the decrease of the intracellular glycogen content,indicating that PAOs can be re-cultivated through regulating the influent P/C ratio,once GAOs had dominated the EBPR system.While,the DGGE profiles showed that the dominant species of PAOs re-cultivated from the GAOs-dominated system was different greatly from both the GAOs-dominated system and the initial PAOs-dominated system. |
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| Keywords: | enhanced biological phosphorus removal(EBPR) P/C ratio phosphorus accumulating organisms(PAOs) glycogen accumulating organisms(GAOs) PCR-DGGE SBR |
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