利用高通量测序技术分析不同溶解氧条件下硝化 活性污泥的菌群结构特性

为了精确并深入了解溶解氧对活性污泥中硝化微生物菌群结构的影响,以在不同溶解氧条件下富集的硝化活性污泥为研究对象,利用高通量测序分析方法考察不同溶解氧富集硝化活性污泥微生物菌群结构的特性及差异.研究结果表明:对于包含氨氧化菌和亚硝酸氧化菌的硝化活性污泥,低溶解氧可以引起较高的生物多样性,而高溶解氧则更利于硝化功能菌(Proteobacteria菌门的Nitrosomonas菌属、Nitrospirae菌门的Nitrospira菌属)的富集;对于仅包含亚硝酸氧化菌的硝化活性污泥,溶解氧对活性污泥生物多样性影响不大,其中Nitrospirae菌门的Nitrospira菌属更适合低溶解氧条件.另外,不同溶解氧条件同样引起了硝化活性污泥中非硝化功能微生物(Bacteriodetes菌门、Chloroflexi菌门、Acidobacteria菌门、Anerolineaceae菌属、Dokdonella菌属、Ferruinibacter菌属等)的菌群结构差异.

Effect of Dissolved Oxygen on Microbial Community of Nitrify Activated Sludge Based on High-throughput Sequencing Technology

To further investigate the effect of dissolved oxygen ( DO ) on microbial community of nitrify bacteria in the activated sludge, the microbial community of enriched nitrify sludge under the different DO level was analyzed by high-throughput sequencing technology. Results show that for nitrify sludge including both ammonium oxidizing bacteria ( AOB ) and nitrite oxidizing bacteria ( NOB ) , low-DO condition increase the microbial diversity, and high-DO enriches the nitrifying functional bacteria ( Nitrosomonas, Nitrospira ) . For nitrify sludge only including NOB, there is no obvious difference of microbial diversity between low-DO condition and high-DO condition, and Nitrospira prefers to grow under the low-DO condition. Moreover, different DO conditions also affect the microbial community of other bacteria except nitrifyer.