| Determination of contents of eight alkaloids in fruits of Macleaya cordata （Willd） R. Br. from different habitats and antioxidant activities of extracts |
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| 引用本文： | 钟明,黄可龙,曾建国,黎霜,张丽.Determination of contents of eight alkaloids in fruits of Macleaya cordata （Willd） R. Br. from different habitats and antioxidant activities of extracts[J].中南工业大学学报(英文版),2010,17(3):472-479. |
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| 作者姓名： | 钟明 黄可龙 曾建国 黎霜 张丽 |
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| 作者单位： | ZHONG Ming(School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;Hunan Engineering Research Center of Botanical Extract, Changsha 410301, China;Department of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology,Yueyang 414006, China);HUANG Ke-long,LI Shuang(School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China);ZENG Jian-guo(Hunan Engineering Research Center of Botanical Extract, Changsha 410301, China);ZHANG Li(Department of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology,Yueyang 414006, China) |
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| 基金项目： | 国家自然科学基金，the International Cooperation Project of Ministry of Science and Technology of China |
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| 摘 要： |
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| 关 键 词： | 生物碱含量 提取物 博落回 抗氧化性 斑鸠 产地 高效液相色谱法 超声波辅助提取 |
| 收稿时间： | 25 March 2011 |
Determination of contents of eight alkaloids in fruits of Macleaya cordata (Willd) R. Br. from different habitats and antioxidant activities of extracts |
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| Ming Zhong, Ke-long Huang, Jian-guo Zeng, Shuang Li and Li Zhang.Determination of contents of eight alkaloids in fruits of Macleaya cordata (Willd) R. Br. from different habitats and antioxidant activities of extracts[J].Journal of Central South University of Technology,2010,17(3):472-479. |
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| Authors: | Ming Zhong Ke-long Huang Jian-guo Zeng Shuang Li and Li Zhang |
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| Affiliation: | [1]School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China [2]Hunan Engineering Research Center of Botanical Extract, Changsha 410301, China [3]Department of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414006, China |
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| Abstract: | A reliable ultrasound-assisted extraction (UAE) method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata (Willd) R. Br. was developed. The optimization conditions of UAE were obtained by using Box-Behnken design of response surface methodology. Chromatography was carried out using a Kromasil C18 column by gradient elution with 0.1% phosphoric acid aqueous solution for HPLC-UV. All calibration curves showed good linear correlation coefficients (R2>0.999 6) and recoveries (from 97.3% to 104.9%) were acceptable. l,l-diphenyl-2-picrylhydrazyl (DPPH) method was employed to test the antioxidant activity of the extract from the samples. The proposed method was successfully applied to quantifying eight components in nine samples of M.cordata, and significant variations of alkaloid contents and antioxidant activity of the samples from different habitats were demonstrated. It presents a powerful proof for the selection of the best sources to extract eight kinds of alkaloids. |
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| Keywords: | Macleaya cordata (Willd) R Br alkaloid ultrasound-assisted extraction antioxidant activity HPLC-UV response surface methodology |
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