| 多重PCR法检测食品中大肠杆菌O157 |
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| 引用本文： | 李睿,戴诗皎,杨敬镜,瞿敏.多重PCR法检测食品中大肠杆菌O157[J].武汉工业学院学报,2010,29(3):1-3. |
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| 作者姓名： | 李睿 戴诗皎 杨敬镜 瞿敏 |
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| 作者单位： | 武汉工业学院,生物与制药工程学院,湖北,武汉,430023 |
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| 摘 要： | 以大肠杆菌O157的stx1、stx2、wzy基因为靶基因,建立了一种新的多重PCR检测方法。PCR扩增结果与各参考菌株基因型一致。PCR结果表明,wzy基因特异性强,可作为检测O157菌株的标志基因,且为开发大肠杆菌O157的分子检测技术提供新的研究思路。
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| 关 键 词： | 大肠杆菌O157 多重PCR stx1 stx2 wzy |
A multiplex PCR system for the detection of Escherichia coli O157 in food |
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| LI Rui,DAI Shi-jiao,YANG Jing-jing,QU Min.A multiplex PCR system for the detection of Escherichia coli O157 in food[J].Journal of Wuhan Polytechnic University,2010,29(3):1-3. |
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| Authors: | LI Rui DAI Shi-jiao YANG Jing-jing QU Min |
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| Affiliation: | LI Rui,DAI Shi-jiao,YANG Jing-jing,QU Min(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China) |
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| Abstract: | A new multiplex PCR was developed to detect Escherichia coli O157 by the amplification of stx1,stx2 and wzy genes.It detected the presence of the three target genes in a manner consistent with the known genotype of each reference strain.The PCR amplification result demonstrated that wzy gene was O157 serotype specific,and could be used for the differentiation of O157 and non-O157 serotypes.This study offered a new method for the detection of Escherichia coli O157. |
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| Keywords: | stx1 stx2 wzy |
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