基于三维图像处理的心肌细胞中Ca~(2+)浓度研究

心肌细胞中Ca2+浓度的空间和时间分布,对于建立心肌细胞节律运动电路模型提供离子特性参数有很大帮助。提出了一种幼鼠心肌细胞中Ca2+浓度分布分析的新方法。对激光共聚焦显微镜(LSCM)采集的幼鼠心肌细胞序列图像进行图像处理、三维重建、细胞分割、距离变换,从细胞膜向内进行分层剥离,得到心肌细胞中Ca2+浓度的空间和时间分布。该方法极大地方便生物研究和医学诊断,基于三维距离变换研究Ca2+浓度时间空间分布的方法也可运用于类似的生物医学研究。

Study of Ca2+ concentrations in cardiac myocytes based on 3-D image processing

The spatio-temporal distribution of Ca2 concentrations in a cardiac myocyte is helpful to get the parameters for circuit modeling of cardiac mycoyte rhythmic motion.A new method for studying the distribution of Ca2 concentrations in cardiac myocytes was proposed.The sequence images of a cardiac myocyte sampled by Laser Scanning Confocal Microscopy(LSCM) were processed,3-D reconstructed,segmented,and distance transformed to divide the myocardial cell into shells from the membrane toward inside,and the spatio-temporal distribution of the Ca2 concentration in the cardiac myocyte was got.3-D medical image processing is convenient to biology research and medical diagnosis,and 3-D distance transform method can also be applied to the analogous biomedical researches.