| 基于C8051F020的多通道手持式PCR扩增仪的设计 |
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| 引用本文： | 丁国杰,赵湛,彭年才,李红东,于留波.基于C8051F020的多通道手持式PCR扩增仪的设计[J].传感技术学报,2010,23(2):158-161. |
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| 作者姓名： | 丁国杰 赵湛 彭年才 李红东 于留波 |
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| 作者单位： | 中国科学院电子学研究所传感技术国家重点实验室,北京,100190;西安天隆科技有限公司,西安,710043 |
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| 基金项目： | 中国高技术研究发展计划(863)项目资助(2007AA042104) |
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| 摘 要： | 设计旨在研制一种新型的手持式PCR扩增仪,利用微电子机械系统(MEMS)技术制作PCR微加热芯片,采用聚二甲基硅氧烷(PDMS)材料制作一次性使用的微反应腔阵列,以C8051F020微控制器为核心搭建一个可以同时进行多路扩增的温控系统。它从根本上克服了传统台式PCR扩增仪的缺点,具有体积小、重量轻、反应速率快、节省反应试剂等诸多优点。
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| 关 键 词： | 聚合酶链式反应 手持式 微电子机械系统 聚二甲基硅氧烷 |
The Design of Multiplex Handheld PCR Amplification Based on C8051F020 |
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| DING Guojie,ZHAO Zhan,PENG Niancai,LI Hongdong,YU Liubo.The Design of Multiplex Handheld PCR Amplification Based on C8051F020[J].Journal of Transduction Technology,2010,23(2):158-161. |
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| Authors: | DING Guojie ZHAO Zhan PENG Niancai LI Hongdong YU Liubo |
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| Affiliation: | 1.The State Key Laboratory of Transducer Technology;Institute of Electronics;Chinese Academy of Sciences;Beijing 100190;China 2.Xian Tian Long Science and Technology Corporation;Xi'an 710043;China |
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| Abstract: | A new type handheld PCR amplification is designed,in which PCR microchip is fabricated with MEMS technology and the one-off microarray is made of PDMS material.C8051F020 microcontroller is made as a core to build a temperature-controlled system,which can carry out several amplifications synchronously.It can overcome the defects of traditional desktop PCR amplification and its advantages include small volume,light weight,fast reaction velocity and saving reaction reagent. |
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| Keywords: | PCR (Polymerase Chain Reaction) handheld MEMS PDMS |
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