基于信号扩增的荧光技术检测 MicroRNAs 的研究进展

基于信号扩增的荧光技术结合了荧光检测与扩增技术两者的优点，是一种拥有广阔发展前景的生化分析 方法。因其具有扩增效率高、信号易于读取和实时分析等优点，正成为核酸检测中的一个研究热点。文中综述了近年来基于信号扩增的荧光技术应用于 microRNAs 检测的最新进展，包括链置换扩增、滚环扩增、基于外切酶的信号扩增、基于双链特异性核酸酶的信号扩增和无酶信号扩增等，并对今后的发展方向进行了展望。

Progress in Signal Amplification-Based Fluorescence Methoeds for MicroRNAs Assay

Signal amplification-based fluorescent methods combine the advantages of both fluorescence detection and signal amplification strategy. Recently, they have gained increasing attention in nucleic acid detection due to their high efficiency, easy read-out, and real-time monitoring. In this review, we summarize the recent progress in signal amplification-based fluorescent methods for microRNAs assay, and focus on five categories including strand displacement amplification (SDA), rolling circle amplification (RCA), exonuclease-assisted signal amplification, duplex specific nuclease (DSN)-assisted signal amplification, and enzyme-free signal amplification. We highlight their future direction as well.