| 基于微流控芯片的钙离子浓度检测系统的实现 |
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| 引用本文： | 柳葆,张思祥,程景萌,周围.基于微流控芯片的钙离子浓度检测系统的实现[J].自动化与仪表,2011,26(1). |
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| 作者姓名： | 柳葆 张思祥 程景萌 周围 |
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| 作者单位： | 河北工业大学现代测控技术研究所,天津,300130 |
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| 摘 要： | 在细胞内物质定量分析中引入微流控芯片.利用微流控芯片完成细胞的培养、染色、试剂的进给等生物实验功能.设计了用于细胞内钙离子浓度检测的微流控荧光检测系统.通过双波长激发,利用荧光检测系统完成荧光强度和图像的采集.同时研究比值荧光法,计算出定量检测的钙离子浓度.实验结果表明,此检测装置可靠性高,检测结果准确.这一研究,提供了一种细胞检测新手段.为细胞研究提供更加便捷的细胞培养、检测、试剂进给一体化检测装置.
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| 关 键 词： | 微流控芯片 细胞检测 钙离子浓度 荧光测量 |
Implementation of Measurement System of Concentration of Ca2+ Based on Microfluidic Chip |
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| LIU Bao,ZHANG Si-xiang,CHENG Jing-meng,ZHOU Wei.Implementation of Measurement System of Concentration of Ca2+ Based on Microfluidic Chip[J].Automation and Instrumentation,2011,26(1). |
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| Authors: | LIU Bao ZHANG Si-xiang CHENG Jing-meng ZHOU Wei |
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| Affiliation: | LIU Bao,ZHANG Si-xiang,CHENG Jing-meng,ZHOU Wei(Research Institute of Modern Measurement and Control Technology,Hebei University of Technology,Tianjin 300130,China) |
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| Abstract: | Microfluidic chip has been introduced to the quantitative analysis of materials within cells.Biotic experiments such as cell fostering,staining,and injecting reagents can be implemented on the designed microfluidic chip.This paper presents a microfluidic fluorescence detection system to measure the concentration of Ca2+ in cells.By dual wavelength light excite,the fluorescence signal and image respectively were captured by fluorescence detection system.The concentration of Ca2+ in cells can be calculated by... |
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| Keywords: | microfluidic chip cell detection the concentration of calcium ion(i) fluorescence measurements |
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