High-throughput automation design considerations for biotechnology processes involving RNA purification protocols using multi-centrifuge bioseparation steps |
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Authors: | Aura-Maria Cardona Zvi Roth Chingping Han |
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Affiliation: | 1. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;2. State Key Laboratory of Chemical Engineering (Tianjin University), Tianjin 300072, China |
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Abstract: | Design of an automation line is a multi-objective optimization problem involving throughput, yield, floor space and cost constraints. The paper examines the feasibility of a computer-aided automation design for biotechnology applications using Arena? software. A generic case study chosen for this study involves a sequence of steps in a preparation process of RNA from tissue-cultured cells. These steps involve repetitive usage of centrifuging operations to perform separation of biochemical substances. A sample must be loaded onto a centrifuge by a pick-and-place device (typically a robot manipulator). Consecutive centrifuging steps may involve multiple centrifuges as well as robots, or some measure of equipment sharing. The paper proposes a unified simplified cost model for all design objectives (throughput, space utilization, process capital and operational cost) and a quantitative selection criterion to allow for an optimal automation design. |
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