| 基于磁性纳米颗粒的化学发光酶免疫分析用于日本血吸虫抗体的检测 |
| |
| 引用本文： | 青宪,楚霞.基于磁性纳米颗粒的化学发光酶免疫分析用于日本血吸虫抗体的检测[J].化学传感器,2008,28(3):21-27. |
| |
| 作者姓名： | 青宪 楚霞 |
| |
| 作者单位： | 1. 湖南师范大学化学化工学院,湖南长沙,410081
2. 湖南师范大学化学化工学院,湖南长沙,410081;湖南大学化学与生物传感及化学计量学国家重点实验室,湖南长沙,410082 |
| |
| 摘 要： | 该文提出了一种高灵敏的基于磁性纳米颗粒的化学发光酶免疫分析方法用于感染兔血清中日本血吸虫抗体的检测.日本血吸虫抗原通过N-(3-二甲基氨丙基)-N-乙基碳二亚胺盐(EDC)和N-羟基琥珀酰亚胺(NHS)固定在磁性纳米颗粒的表面,选择性地结合与富集血清中的日本血吸虫抗体,然后与辣根过氧化物酶标记的羊抗兔二抗夹心进行免疫分析.对免疫反应的实验条件如日本血吸虫抗原在磁性纳米颗粒表面的标记浓度,磁性纳米颗粒的用量,抗原与待测抗体的反应时间及辣根过氧化物酶标记二抗的浓度进行了优化.发光强度与感染兔血清的稀释比在1:10000～1:100的范围内成良好的线性关系,检测下限为1:13442.
|
| 关 键 词： | 化学发光 磁性纳米颗粒 日本血吸虫抗体 |
Chemiluminescent enzyme immunoassay based on magnetic nanoparticles for the detection of schistosoma japonicum antibody |
| |
| Qing Xian,Chu Xia.Chemiluminescent enzyme immunoassay based on magnetic nanoparticles for the detection of schistosoma japonicum antibody[J].Chemical Sensors,2008,28(3):21-27. |
| |
| Authors: | Qing Xian Chu Xia |
| |
| Affiliation: | Qing Xian1,Chu Xia1,2 (1. Chemistry , Chemical Engineering College,Hunan Normal University,Changsha 410081,China) ( 2. State Key Laboratory for Chem/Biosensing , Chemometrics,Hunan University,Changsha 410082,China) |
| |
| Abstract: | A novel chemiluminescent enzyme immunoassay based on magnetic nanoparticles (MNPs) has been developed for highly sensitive and rapid detection of schistosoma japonicum antibody (SjAb) in infected rabbit serum (IRS). Schistosoma japonicum antigen (SjAg) was immobilized onto MNPs using the N- (3- dimethylaminopropy)- N'- ethylcarbodiimidehydrochloride (EDC) and N- hydroxysuccinmide (NHS). After reacting with SjAb, the horseradish peroxidase- conjugated goat anti- rabbit IgG(HRP- IgG) was bound to the surface ... |
| |
| Keywords: | chemiluminescence magnetic nanoparticles schistosoma japonicum antibody |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
