用于无创检测皮下葡萄糖的新型生物传感器研究

以无创检测人体血糖为应用需求,采用高灵敏度锇氧化还原聚合物修饰在薄膜电极上,并通过戊二醛交联法固定酶分子制备成新型生物传感器。实验结果表明:在0~700μmol/L的葡萄糖标准浓度范围内,传感器灵敏度为23.955 nA/(μmol.L-1),最低检测限为0.3μmol/L,相关系数为0.999;在标准皮下葡萄糖浓度0~19mmol/L浓度范围内,被抽取出的葡萄糖电流响应值与皮下葡萄糖的浓度成线性关系,线性相关系数为0.994,灵敏度为4.03 nA/(mmol.L-1);单只传感器对100μmol/L葡萄糖检测的精度为4.07%(n=10),不同传感器之间对100μmol/L葡萄糖测量的精度为3.22%(n=10),在4℃条件下,传感器的寿命可达450 d。

Research on new biosensor for non-invasive detection of subcutaneous glucose

A new sensitive biosensor is formed by the immobilization of Os-complex redox polymer and glucose oxidase on the electrode surface to determination glucose in noninvasive way.Glucose biosensor exhibits analytic sensitivity of 23.955nA /(μmol·L-1) with the correlation coefficient up to 0.999 in the relevant concentration range of 0~700μmol/L.The limit of detection for glucose is 0.3μmol/L.The biosensor exhibits sensitivity of 4.03nA/(mmol·L-1) for subcutaneous glucose.In the relevant concentration range of 0~19mmol/L,the correlation coefficient is 0.994.Relative standard deviations are 4.07% and 3.22% for within-run precision and between-run precision(n=10),respectively in 100μmol/L glucose detection.The biosensor life is about 450d stored at 4℃.