New system for real time study of in vivo migration and differentiation of stem cells |
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Authors: | Siddiqul Haque G��nter R Fuhr |
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Affiliation: | (1) Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, Ensheimer Str. 48, 66386 St Ingbert, Germany;(2) Fraunhofer Institute for Biomedical Engineering, Ensheimer Str. 48, 66386 St Ingbert, Germany |
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Abstract: | Cell therapy is a promising and emerging field for the treatment of human diseases. However, to understand and optimize cell
therapy, the inability to track the cell transplants in vivo remains a major problem. Most cell transplantation techniques
involve the use of histological analysis to evaluate cell transfection, proliferation, and migration after sacrifice. In this
literature, for the first time an in vivo model has been developed to study stem cells. Explantation culture of chicken embryo
into surrogate shells has been modified for high resolution and long term imaging. A special long distance fluorescence microscope
and micromanipulation systems has been developed for in vivo application specially aiming for the injection and tracking of
fluorescence labeled cells into chicken embryo and track them. By using the developed system, it was possible to image the
whole period of embryonic development of chicken embryo. |
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