| Thermus thermophilus木糖异构酶复合物模型的建立与分析 |
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| 引用本文： | 许伟,严明,丁莉,李艳,许琳.Thermus thermophilus木糖异构酶复合物模型的建立与分析[J].计算机与应用化学,2006,23(6):531-534. |
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| 作者姓名： | 许伟 严明 丁莉 李艳 许琳 |
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| 作者单位： | 南京工业大学制药与生命科学学院,江苏,南京,210009 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | Thermus thermophilus木糖异构酶在高果糖浆的工业生产及木糖发酵重组菌的构建方面具有极其广阔的应用前景．本文主要运用结构分析和分子对接及计算软件,确定了Mg~2及木糖异构酶天然底物D-木糖在1BXB晶体结构中的定位,构建出1BXB复合物模型并进行评价与分析．通过对1BXB复合物模型1与1XIC的结构进行比较,发现所构建模型中D-木糖周围的残基在木糖异构酶中高度保守,并且D-木糖的定位与取向与1XIC中底物分子基本一致．
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| 关 键 词： | 嗜热栖热菌 木糖异构酶 结构 对接 |
| 文章编号： | 1001-4160（2006）06-531-534 |
| 收稿时间： | 2005-12-17 |
| 修稿时间： | 2005-12-172006-06-10 |
Modeling and Analysis the Complex of Xylose Isomerase with D - Xylose from Thermus Thermophilus |
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| Xu Wei,Yan Ming,Ding Li,Li Yan,Xu Lin.Modeling and Analysis the Complex of Xylose Isomerase with D - Xylose from Thermus Thermophilus[J].Computers and Applied Chemistry,2006,23(6):531-534. |
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| Authors: | Xu Wei Yan Ming Ding Li Li Yan Xu Lin |
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| Affiliation: | College of Life Science and Pharmacy, Nanjing University of Chemical Technology, Nanjing, 210009, Jiangsu Province, China |
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| Abstract: | Xylose isomerase from Thermus thermophilus is widely used in the production of high-fructose corn syrup and the construction of xylose utilizing recombinant strains.In this paper,the positions of Mg ions and D-xylose in 1BXB were established by structure analysis,molecular docking and computing.The Complex of 1BXB with D-Xylose was modeled and analyzed. Comparison structure of IBXB complex and 1XIC,we found that the overall structure of them showed high similarity.The residues around D-xylose were highly conserved in xylose isomerase,and the orientation and positions of D-xylose were very similar in both structures.This suggests they have similar function and close genetic relationship. |
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| Keywords: | Thermus thermophilus Xylose Isomerase structure docking |
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