| 甲壳素固定化蜗牛酶对壳聚糖的降解行为 |
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| 引用本文： | 张剑,荆迎军,吴兆亮.甲壳素固定化蜗牛酶对壳聚糖的降解行为[J].高分子材料科学与工程,2009(9). |
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| 作者姓名： | 张剑 荆迎军 吴兆亮 |
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| 作者单位： | 河北工业大学化工学院生物工程系; |
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| 基金项目： | 河北省科学技术研究与发展指导计划(07215507) |
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| 摘 要： | 采用天然高分子聚合物甲壳素作载体,以戊二醛为交联剂,对蜗牛酶进行固定化。研究了酶量、戊二醛浓度、pH和溶剂对蜗牛酶固定化的影响。结果表明,0.5g甲壳素与0.8%的戊二醛交联后,与用双蒸水配制的pH为5.6的酶液混合制备的固定化酶活性最高。用优化条件下制备的固定化酶降解壳聚糖,先调pH为4.0,360min后调pH为4.8使得平均降解速率最大。当壳聚糖质量百分数为0.3%时,初始反应速率最大,并提出了新的经验模型描述壳聚糖的降解过程。用该固定化酶连续降解壳聚糖70d,酶活为原来的98%,降解性能保持稳定。
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| 关 键 词： | 壳聚糖降解 固定化酶 蜗牛酶 甲壳素 |
Hydrolysis of Chitosan with Chitin Immobilized Snailase |
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| ZHANG Jian,JING Ying-jun,WU Zhao-liang.Hydrolysis of Chitosan with Chitin Immobilized Snailase[J].Polymer Materials Science & Engineering,2009(9). |
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| Authors: | ZHANG Jian JING Ying-jun WU Zhao-liang |
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| Affiliation: | ZHANG Jian,JING Ying-jun,WU Zhao-liang(Department of Bioengineering,School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300130,China) |
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| Abstract: | Snailase was immobilized on chitin by glutaraldehyde through cross-link reaction. The effects of amount of enzyme,glutaraldehyde concentration,pH,and solvent on the immobilized enzyme were assayed. The results show that after cross-link reaction between 0.5 g of chitin and 0.8% of glutaraldehyde,the immobilized snailase shows the highest activity by mixing the snailase solution (made by double distilled water and adjusted to pH 5.6) and cross-linked chitin. The degradation rate on average by the immobilized... |
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| Keywords: | chitosan degradation immobilized enzyme snailase chitin |
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