新型壳聚糖/PBLG-co-PGA复合膜的研究

采用壳聚糖和聚谷氨酸苄酯-co-聚谷氨酸(PBLG-co-PGA)通过1-乙基-3-(3-二甲基氨基丙基)碳化二亚胺(EDC)与N-羟基琥珀酰亚胺(NHS)交联得到复合膜.采用FTIR和XRD对复合膜的结构进行了表征,同时对复合膜的吸水率进行了研究.研究结果表明,复合膜中壳聚糖和PBLG-co-PGA分子之间成功地发生了交联反应,二者之间的交联与氢键作用改变了二者原有的结晶结构,这对复合膜的性能有着重要的影响.以复合膜为载体培养软骨细胞发现,复合膜与壳聚糖膜相比,具有更高的细胞粘附率,预示着该复合膜是一种具有良好应用前景的生物医用材料.

Study on the novel composite film composed of chitosan and poly(γ-benzyl L-glutamate)-co-poly(glutamic acid)

The novel composite film was prepared by chitosan and poly (γ-benzyl L-glutamate)-co-poly (glutamic acid),which were crosslinked by nontoxic crosslinking reagents of 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS).The structure of the composite film was characterized by FTIR and XRD and the properties of water absorbency and in vitro biocompatibility were studied at the same time.The results indicated that the chitosan and PBLG-co-PGA were successfully crosslinked and the intrinsic crystal structures of the both materials were destroyed because of the intermolecular crosslinking and hydrogen-band action.The change of crystalline structures had an effect on the properties of the film.The results of culturing chondrocytes showed that there was the higher cell adherent ratio and the better cell configuration on the composite film than the pure chitosan film,which indicated that the composite film could become a promising material used in biomedical field.