茶多酚/壳聚糖/海藻酸钠纳米微球的制备

目的探讨各因素对制备茶多酚/壳聚糖/海藻酸钠纳米微球载药率、包埋率的影响,研究纳米微球体外释放行为,为后期缓释抗菌膜的制备提供基础。方法采用单因素实验、正交实验考察海藻酸钠溶液浓度、壳聚糖溶液浓度、CaCl_2溶液浓度、茶多酚溶液浓度对纳米微粒载药率、包封率的影响,并考察其体外释放率。结果当海藻酸钠溶液、壳聚糖溶液、CaCl_2溶液、茶多酚溶液的质量浓度分别为15,10,15,0.8 mg/m L时,该工艺条件下制备的纳米微粒载药率为22.71%,包封率为61.38%,且粒径集中在500 nm左右,有较好的缓释效果。结论所得的最佳工艺制备条件为后期做缓释抗菌膜打下良好基础。

Preparation of Polyphenols/Chitosan/Alginate Nano-microspheres

The work aims to study the effects of various factors on the loading efficiency and encapsulation efficiency of polyphenol/chitosan/alginate nano-microspheres and research the in vitro release rate of nano-microspheres, so as to provide the preliminary basis for the preparation of slow release antibacterial film at later periods. Through the single factor experiment and orthogonal test, the effects of concentration of alginate, chitosan, CaCl2, polyphenol solutions on the loading efficiency and encapsulation efficiency of nano-particles were investigated, and the in vitro release rate was studied. When the concentrations of alginate, chitosan, CaCl2, polyphenol solutions were 15, 10, 15 and 0.8 mg/mL, the loading efficiency of nano-particles prepared under such process condition was 22.71%, the encapsulation efficiency was 61.38% and the particle size was mainly around 500 nm; the results showed that microspheres had a good sustained release effect. The optimal process preparation conditions obtained lays a good foundation for the preparation of slow release antibacterial film at later periods.