| A microfluidic approach for development of hybrid collagen-chitosan extracellular matrix-like membranes for on-chip cell cultures |
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| 作者姓名： | Erica Rosella Nan Jia Diego Mantovani Jesse Greener |
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| 作者单位： | Laboratory, Department of Chemistry, Laval University, Québec, QC, Canada;Laboratory of Biomaterials and Bioengineering, CRC-1, Dept.Min-Met-Materials Eng & CHU de Quebec, Research Center, Laval University, Québec, QC,Canada;Laboratory, Department of Chemistry, Laval University, Québec, QC, Canada;Laboratory of Biomaterials and Bioengineering, CRC-1, Dept.Min-Met-Materials Eng & CHU de Quebec, Research Center, Laval University, Québec, QC,Canada;CHU de Quebec Research Centre, Laval University, 10 rue de l'Espinay, Québec, QC G1L 3L5, Canada;Laboratory, Department of Chemistry, Laval University, Québec, QC, Canada;CHU de Quebec Research Centre, Laval University, 10 rue de l'Espinay, Québec, QC G1L 3L5, Canada |
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| 基金项目： | funding from Fonds de recherche du Québec–Nature et technologies(FRQNT);Natural Sciences and Engineering Research Council of Canada(NSERC);the Canadian Foundation for Innovation(CFI)for financial support for this work。 |
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| 摘 要： | To advance organ-on-a-chip development and other areas befitting from physiologically-relevant biomembranes,a microfluidic platform is presented for synthesis of biomembranes during gelation and investigation into their role as extracellular matrix supports.In this work,high-throughput studies of collagen,chitosan,and collagen-chitosan hybrid biomembranes were carried out to characterize and compare key properties as a function of the applied hydrodynamic conditions during gelation.Specifically,depending on the biopolymer material used,varying flow conditions during biomembrane gelation caused width,uniformity,and swelling ratio to be differently affected and controllable.Finally,cell viability studies of seeded fibroblasts were conducted,thus showing the potential for biological applications.
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| 关 键 词： | MATRIX SWELLING showing |
A microfluidic approach for development of hybrid collagen-chitosan extracellular matrix-like membranes for on-chip cell cultures |
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| Erica Rosella,Nan Jia,Diego Mantovani,Jesse Greener.A microfluidic approach for development of hybrid collagen-chitosan extracellular matrix-like membranes for on-chip cell cultures[J].Journal of Materials Science & Technology,2021,63(4):54-61. |
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| Authors: | Erica Rosella Nan Jia Diego Mantovani Jesse Greener |
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| Abstract: | To advance organ-on-a-chip development and other areas befitting from physiologically-relevant biomembranes, a microfluidic platform is presented for synthesis of biomembranes during gelation and investigation into their role as extracellular matrix supports. In this work, high-throughput studies of collagen, chitosan, and collagen-chitosan hybrid biomembranes were carried out to characterize and compare key properties as a function of the applied hydrodynamic conditions during gelation. Specifically, depending on the biopolymer material used, varying flow conditions during biomembrane gelation caused width, uniformity, and swelling ratio to be differently affected and controllable. Finally, cell viability studies of seeded fibroblasts were conducted, thus showing the potential for biological applications. |
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| Keywords: | Extracellular matrix Membrane Natural polymers Biomaterials Microfluidics Fibroblasts |
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