| 水杨酸对喜树细胞次级代谢的诱导作用 |
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| 引用本文： | 于放,张冬艳,白凤武,安利佳.水杨酸对喜树细胞次级代谢的诱导作用[J].高技术通讯,2005,15(1):70-73. |
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| 作者姓名： | 于放 张冬艳 白凤武 安利佳 |
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| 作者单位： | 大连理工大学环境与生命学院生物科学与工程系,大连,116023 |
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| 基金项目： | 国家自然科学基金 (2 0 1760 5 8)资助项目 |
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| 摘 要： | 将水杨酸(SA)应用于喜树细胞培养体系中。通过添加不同浓度的水杨酸，研究了它对喜树细胞过氧化物酶(POD)活力、超氧化物歧化酶(SOD)活力、可溶性蛋白含量、细胞活力以及细胞生长和代谢产物积累的影响。结果表明，SA可以提高POD和SOD的活力以及可溶性蛋白的含量，增强喜树细胞的次级代谢作用，有利于代谢产物喜树异碱A和喜树异碱B的合成。
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| 关 键 词： | 细胞 喜树 次级代谢 诱导作用 SA 水杨酸 代谢产物 活力 可溶性蛋白含量 POD |
Effect of Salicylic Acid on Secondary Metabolism in Cell Suspension Culture of Camptotheca acuminata |
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| Yu Fang,Zhang Dongyan,Bai Fengwu,An Lijia.Effect of Salicylic Acid on Secondary Metabolism in Cell Suspension Culture of Camptotheca acuminata[J].High Technology Letters,2005,15(1):70-73. |
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| Authors: | Yu Fang Zhang Dongyan Bai Fengwu An Lijia |
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| Abstract: | The activity of peroxidase (POD), superoxide dismutase (SOD), the content of soluble protein, and the biosynthesis of Isocamptothecin A and Isocamptothecin B in suspension cell culture of Camptotheca acuminata exposed to various concentrations of salicylic acid (SA) were investigated. The results indicated that the addition of SA was followed by an increase in POD and SOD activity and it also promoted the content of soluble protein. The secondary metabolism of Camptotheca acuminata cells was enhanced by addition of SA, and it is of benefit to accumulation of Isocamptothecin A and Isocamptothecin B in suspension cell culture of Camptotheca acuminata. |
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| Keywords: | Camptotheca acuminata salicylic acid plant cell culture Isocamptothecin A Isocamptothecin B |
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