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麻疯树核糖体失活蛋白的分离纯化和作用机制研究
引用本文:林娟,颜钫,唐琳,陈放.麻疯树核糖体失活蛋白的分离纯化和作用机制研究[J].高技术通讯,2002,12(11):36-40.
作者姓名:林娟  颜钫  唐琳  陈放
作者单位:四川大学生命科学学院,成都,610064
摘    要:从麻疯树种子中分离纯化出一种核糖体失活蛋白,用SDS-PAGE测定其相对分子量为28.2kD,IEF-PAGE计算其等点为8.54左右。体外抑制实验表明,麻疯树核糖体失活蛋白对兔网织红细胞裂解液的蛋白质生物合成有较强的抑制活性。当麻疯树核糖体失活蛋白作用于大鼠肝核糖体经苯胺处理后,在聚丙烯酰胺的尿素变性胶电泳图上能观察到450bp左右的特征性核酸条带,证明麻疯树核糖体失活蛋白是一种RNA N-糖苷酶,从而首次阐明了麻疯树糖体失活蛋白失活核糖体的分子机制。

关 键 词:作用机制  麻疯树  核糖体失活蛋白  分离纯化  RNA  N-糖苷酶  相对分子量

Isolation, Purification and Functional Investigation on the N-glycosidase Activity of Curcin from the Seeds of Jatropha curcas
Lin Juan,Yan Fang,Tang Lin,Chen Fang.Isolation, Purification and Functional Investigation on the N-glycosidase Activity of Curcin from the Seeds of Jatropha curcas[J].High Technology Letters,2002,12(11):36-40.
Authors:Lin Juan  Yan Fang  Tang Lin  Chen Fang
Abstract:A ribosome inactivating protein curcin was isolated from the seeds of Jatropha curcas. The results of SDS PAGE indicate that its molecular weight was around 28.2 kD, and the results of IEF PAGE indicate its isoelectric point was 8.54. The curcin strongly inhibits protein synthesis in ribbit reticulocyte lysate system. When rRNAs were simultanous treated with curcin and aniline, a characteristic fragment of approximant 450 bp was obtained, which reveals that curcin has the activity of RNA N glycosidase indeed. This study reveals for the first time that the functional mechanism of curcin at molecular level.
Keywords:Jatropha curcas    Curcin  Isolation and purification  RNA N  glycosidase  
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